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Biotoxicity assay of Escherichia coli against Tribolium castaneum (Paperback)
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Biotoxicity assay of Escherichia coli against Tribolium castaneum (Paperback)
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Pathogenic Escherichia coli is found in the contaminated raw meat,
raw milk, raw vegetables, contaminated water. The present work was
based on the entomopathogenic effect of E.coli against stored grain
pest Tribolium castaneum. Microbial screening of pathogenic
bacteria was done by using different media such as TSB media,
MacConkey media and Novobiocin media. Rearing of Tribolium
castaneum was done and different stages of Tribolium castaneum such
as larvae, pupae and adult were observed. Bioassay technique was
applied for the detection of effect of E.coli against the Tribolium
castaneum. The Bioassay was applied in two groups e.g. Control
group and Experimental group. The pellet of grown culture of E.coli
sample was mixed in the diet of Tribolium castaneum. The effect of
E.coli was observed against 2nd Instar larvae of Tribolium
castaneum upto three days and seven days.The mortality rate in both
control group and Experimental group was approximately 10%. The
results had shown that E.coli did not have any effect against
Tribolium castaneum."
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