Craniofacial Development - Methods and Protocols (Paperback, 1st ed. 2022)

This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Craniofacial Development: Methods and Protocols aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques.

General

Imprint:	Springer-Verlag New York
Country of origin:	United States
Series:	Methods in Molecular Biology, 2403
Release date:	December 2022
Editors:	Sebastian Dworkin
Dimensions:	254 x 178mm (L x W)
Format:	Paperback
Pages:	313
Edition:	1st ed. 2022
ISBN-13:	978-1-07-161849-3
Categories:	Books > Science & Mathematics > Biology, life sciences > Developmental biology Books > Science & Mathematics > Biology, life sciences > Cellular biology > General Promotions
LSN:	1-07-161849-0
Barcode:	9781071618493

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