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Craniofacial Development - Methods and Protocols (Hardcover, 1st ed. 2022)
Loot Price: R5,499
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Craniofacial Development - Methods and Protocols (Hardcover, 1st ed. 2022)
Series: Methods in Molecular Biology, 2403
Expected to ship within 10 - 15 working days
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This volume explores scientific methodologies currently employed to
integrate observational developmental biology, tissue explant and
cell-based approaches and genetic/molecular technologies to develop
a holistic understanding of craniofacial development. Chapters
guide readers through the use of disparate models to study
formation of the head and face (c. elegans, zebrafish, mouse,
alongside human imaging approaches), together with cell culture,
tissue explant and in vivo cell imaging and analysis techniques. At
the molecular level, chapters include analysing gene expression
using in-situ hybridisation and single-cell RNA-Sequencing
(scRNA-SEQ), as well as genetic modification techniques such as
CRISPR/Cas9-mediated deletion. Written in the format of the highly
successful Methods in Molecular Biology series, each chapter
includes an introduction to the topic, lists necessary materials
and reagents, includes tips on troubleshooting and known pitfalls,
and step-by-step, readily reproducible protocols. Authoritative and
cutting-edge, Craniofacial Development: Methods and Protocols aims
to be a guide in the field of craniofacial development for senior
and new researchers looking to expand their existing research
programs to encompass novel techniques.
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