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Books > Science & Mathematics > Biology, life sciences > Zoology & animal sciences > Vertebrates > Amphibians
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Transgenic Xenopus - Microinjection Methods and Developmental Neurobiology (Hardcover, 1997 ed.)
Loot Price: R2,892
Discovery Miles 28 920
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Transgenic Xenopus - Microinjection Methods and Developmental Neurobiology (Hardcover, 1997 ed.)
Series: Neuromethods, 28
Expected to ship within 10 - 15 working days
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The need to better understand the molecular, b- chemical, and
cellular processes by which a developing neuronal system unfolds
has led to the development of a unique set of experimental tools
and organisms. Special emphasis was devoted to allowing us access,
at the ear- est stages, to the genomic basis underlying the
system's ultimate complexity, as exhibited once its structures are
fully formed. Yet, nerve cells are anatomically, physiolo- cally,
and biochemically diverse. The multitude of d- tinctly different
routes for their development thus makes the developing nervous
system especially intriguing for molecular neurobiologists. In
particular, the demands of modern molecular neuroscience call for
the establishment of efficient yet versatile systems for studying
these c- plex processes. Transgenic embryos of the frog Xenopus
laevis offer an excellent system for approaching neuroscientific
issues. Insertion of foreign genes is performed simply, by mic-
injection under binocular observation; hundreds of in
vitro-fertilized embryos can be microinjected in one experiment.
Embryos develop in tap water, at room t- perature, and within a few
days become independent swimming tadpoles with fully functioning
neuromus- lar systems. Being relatively small, these organisms are
amenable to detailed analyses at the levels of mRNA, protein, and
cell. Their rapid development permits the study of morphogenetic
processes involved in early development, such as myogenesis and
neural induction, as well as those involved in organogenesis and
formation of the brain, the musculature, and the interconnections
between them. Foreign DNA remains predominantly extrachromosomal.
General
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