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Microbial Transposon Mutagenesis - Protocols and Applications (Hardcover, 1st ed. 2019)
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Microbial Transposon Mutagenesis - Protocols and Applications (Hardcover, 1st ed. 2019)
Series: Methods in Molecular Biology, 2016
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This book addresses cutting-edge techniques for researching
transposon mutagenesis, an approach for identifying individual gene
contributions to the phenotypic characteristics of a particular
microorganism. The volume begins with methods for specific
microorganisms and include protocols for individual microorganisms
ranging from pathogens such as Salmonella to Bifidobacterium, a
microorganism considered beneficial to humans and animals. The
final section addresses more general protocols including plasmid
transfer and bioinformatic tools as well as novel applications of
transposon methodologies such as transposon-aided capture of
antibiotic resistant plasmids. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions
to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microbial Transposon Mutagenesis:
Protocols and Applications serves as a valuable reference for
scientists seeking to apply transposon mutagenesis to microbial
genetic analyses and functionality.
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