Updated and revised, this thorough volume provides a selection of
the newest methods, as well as some of the basic methods required
for a mycobacterial research laboratory. Mycobacteria Protocols,
Third Edition guides readers through fractionation and analysis of
macromolecules, from nucleic acids to proteins, complex lipids, and
metabolites. Detailed and comprehensive protocols are provided for
protein and lipid/glycolipid analysis using well-established
methods; these are now complemented by a metabolomics chapter in
which the complement of metabolites can be profiled. Written in the
highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols and tips on troubleshooting and
avoiding known pitfalls. Authoritative and up-to-date, Mycobacteria
Protocols, Third Edition will be a resource both to those working
in the field and to newcomers.
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