This detailed volume focuses on genotyping and validation in
addition to information on how to produce gene edited cells and
animals for research. Future advances in biomedical research will
benefit greatly from the use of precise gene targeting of
transgenes in the genome as CRISPR technology supersedes earlier
methods that relied on random transgene integration, which this
collection reflects. Written for the highly successful Methods in
Molecular Biology series, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step and readily reproducible laboratory protocols, as well
as tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Transgenesis: Methods and Protocols
serves as an ideal guide for researchers working with or working on
genetically modified models.
General
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