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Cells and Culture - Proceedings of the 20th ESACT Meeting, Dresden, Germany, June 17-20, 2007 (Hardcover, 2010)
Loot Price: R4,876
Discovery Miles 48 760
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Cells and Culture - Proceedings of the 20th ESACT Meeting, Dresden, Germany, June 17-20, 2007 (Hardcover, 2010)
Series: ESACT Proceedings, 4
Expected to ship within 12 - 17 working days
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Total price: R4,896
Discovery Miles: 48 960
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Regeneration of tissue to replace damaged or injured tissue is the
goal of t- sue engineering. Biomaterials like polyglycolic acid,
collagen and small-intestinal submuscosa provide a temporary
scaffold to guide new tissue growth and or- nization. Typically,
they need to be biodegradable, showing good cell atta- ment and
proliferation and they should possess appropriate mechanical
properties (Kim et al. , 2000). Synthetic polymers ful ll most of
these requirements but lack cell-adhesion peptides on their surface
to enhance cell attachment. Ce- adhesion peptides are present in
ECM proteins like collagen and elastin. Thus a synthetic polymer
coated with ECM proteins would result in a scaffold that mimics the
natural cellular environment with enhanced cell attachment and p-
liferation. The new bioactive scaffold will be made by combining a
synthetic polymer coated with a layer of recombinant ECM proteins
produced by CHO cells. The rst step consists of identifying
polymers that give best results in terms of CHO cell attachment and
growth. Classical techniques to determine biomass are inappropriate
to evaluate 3-D structures. Thus a screening system based on stable
GFP expressing CHO cells was used to compare the different
scaffolds. Simple uorescent measurement after cell lysis allows
determining cell attachment and p- liferation on synthetic
polymers. Finally CHO cells producing human recombinant collagen I
and elastin were generated. We showed that both proteins are
expressed and secreted by CHO DG44 cells. 2 Materials and Methods
2.
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