This volume details basic and advanced protocols for both stages of
protein engineering: the library design phase and the
identification of improved variants by screening and selection.
Chapters focus on enzyme engineering using rational and
semi-rational approaches. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions
to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Protein Engineering: Methods and
Protocols aims to aid scientists in the planning and performance of
their experiments. The chapter 'Functional Analysis of Membrane
Proteins Produced by Cell-Free Translation' is open access under a
CC BY 4.0 license via link.springer.com.
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