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Basic Cloning Procedures (Paperback, 1998 ed.)
Loot Price: R1,521
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Basic Cloning Procedures (Paperback, 1998 ed.)
Series: Springer Lab Manuals
Expected to ship within 10 - 15 working days
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Experience in laboratory experiments in molecular biology is
important to students, researchers and practioners in the biolo
gical and medical sciences. This book is designed to introduce the
reader to basic methods used in the isolation, cloning and analysis
of genetic material. The text includes theory and fundamental
methods in suffi cient experimental detail which have been
successfully used in our studies of viral genome and eukaryotic
gene regulation. Spe cific protocols together with troubleshooting
tips are given for RT-PCR amplification, gene cloning,
hybridization analysis and sequencing of nucleic acids, PCR-based
site-specific mutagene sis, analysis of protein DNA-specific
interaction, cell-free protein synthesis and product
electrophoretic and immunological analy sis. The methods chosen
have been approved in several interna tional Molecular Biology
Courses and without doubt can be used as a manual in basic
molecular studies of DNA and RNA. The editor is grateful to all the
contributors for their coopera tion and would like to express his
thanks to the publishers, espe cially Dr. J. Lindenborn from
Springer-Verlag, for their coopera tion, patience and assistance
during the preparation of this book. Riga, Latvia V. BERZINS
Contents Chapter 1 cDNA Synthesis and Cloning ... . . . . . . . . .
. . . . . . . . . . . . . 1 JURIS STEINBERGS AND ALEKSANDER
TSIMANIS Chapter 2 DNA Sequencing. . . . . . . . . . . . . . . . .
. . . 22 . . . . . . . . . . . . . . . . ERIKS JANKEVICS Chapter 3
RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase. .
. . . . . . . . . . . . . . . . . . 43 . . . . . . . . . . . . . .
. . ELITA AVOTA AND NORMUNDS LICIS Chapter 4 PCR-Based
Site-Specific Mutagenesis. . . . . . . . . . . 53 . . . . . . . .
JANIS KLOVINS AND VALDIS BERZINS Chapter 5 Analysis of Specific
Protein-DNA Interactions. . . . . . . . . 68 . ."
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