Basil Proteomics (Paperback)

As a part of the systematic and proteomic approach based characterization of plant material with the advantage of producing valuable informations of functional genomics for a general crop improvement programme, leaves of holy medicinal-aromatic plant Tulsi (Ocimum basilicum L.) were used as a source of proteins and a model eukaryotic system. In this study, we developed a reliable protocol for PEG mediated enrichment of low abundant cellular proteins to increase their detection through 2 DE-SDS PAGE. I6% PEG was found to be optimum for the precipitation of Ocimum protein. Further, proteome proteins in different fractions were analysed electrophoretically including 2- DE technique. These protocols may also will helpful in further studies in basil proteomics and other plants also.

General

Imprint:	Lap Lambert Academic Publishing
Country of origin:	Germany
Release date:	September 2012
First published:	September 2012
Authors:	Vikas Sharma • Vikash Singh Jadon
Dimensions:	229 x 152 x 5mm (L x W x T)
Format:	Paperback - Trade
Pages:	80
ISBN-13:	978-3-659-23569-6
Categories:	Books > Science & Mathematics > Biology, life sciences > General
LSN:	3-659-23569-5
Barcode:	9783659235696

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