In this second edition of a widely used classic laboratory manual,
leading experts utilize the tremendous progress and technological
advances that have occurred to create a completely new collection
of not only the major basic techniques, but also advanced protocols
for yeast research and for using yeast as a host to study genes
from other organisms. The authors provide detailed methods for the
isolation of subcellular components-including organelles and
macromolecules, for the basic cellular and molecular analysis
specific for yeast cells, and for the creation of conditional
mutant phenotypes that lend themselves to powerful genome
manipulation. Additional protocols offer advanced approaches to
study genetic interactions, DNA and chromatin metabolism, gene
expression, as well as the foreign genes and gene products in yeast
cells.
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