A collection of standard and cutting-edge techniques for using
Xenopus oocytes and oocytes/egg extracts to reconstitute biological
and cellular processes. These readily reproducible methods take
advantage of the oocyte's impressive protein abundance, its
striking protein translation capacity, and its breathtaking
possibilities for the assembly of infectious viral particles by
single cell injection of multiple RNAs. The authors focus on the
versatility of frog oocytes and egg extracts in cell biology and
signal transduction, and cover all the major uses of
oocytes/extracts as experimental models.
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