During the past decade as the data on gene sequences and
expression patterns rapidly accumulated, cell-free protein
synthesis technology has also experienced a revolution, becoming a
powerful tool for the preparation of proteins for their functional
and structural analysis. In Cell-Free Protein Production: Methods
and Protocols, experts in the field contribute detailed techniques,
the uses of which expand deep into the studies of biochemistry,
molecular biology, and biotechnology. Beginning briefly with basic
methods and historical aspects, the book continues with thorough
coverage of protein preparation methods, the preparation of
proteins that are generally difficult to prepare in their
functional forms, applications of the cell-free technologies to
protein engineering, as well as some methods that are expected to
constitute a part of future technologies. Written in the highly
successful Methods in Molecular Biology series format, the chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and notes on troubleshooting and
avoiding known pitfalls.
Authoritative and cutting-edge, Cell-Free Protein Production:
Methods and Protocols aims to help researchers continue the growth
of the vital exploration of cell-free sciences and technologies in
order to better understand the dynamic lives of cells.
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