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This book presents multiple new and classical methods for studying
the vital poly-ADP-ribose (pADPr) pathway. Beginning with
techniques for the detection and quantification of the product of
poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection
of variation in pADPr production during the cell cycle, the volume
continues with sections on the identification of pADPr protein
acceptors, methods focusing on studying molecular mechanisms of
PARP functions in eukaryotic cells, particularly those involved in
control of DNA repair and oxidative stress, as well as in
expression regulation, approaches to the in vitro reconstitution of
PARP-1 interaction with chromatin, the development and testing of
small molecule PARP inhibitors, and the functions of understudied
members of PARP family. Written for the highly successful Methods
in Molecular Biology series, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Poly(ADP-Ribose) Polymerase: Methods
and Protocols, Second Edition serves as an ideal companion to the
first edition for scientists whose investigations involve this
important pathway. The chapter 'Identifying and Validating
Tankyrase Binders and Substrates: A Candidate Approach' is
published open access under a CC BY 4.0 license.
This book presents multiple new and classical methods for studying
the vital poly-ADP-ribose (pADPr) pathway. Beginning with
techniques for the detection and quantification of the product of
poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection
of variation in pADPr production during the cell cycle, the volume
continues with sections on the identification of pADPr protein
acceptors, methods focusing on studying molecular mechanisms of
PARP functions in eukaryotic cells, particularly those involved in
control of DNA repair and oxidative stress, as well as in
expression regulation, approaches to the in vitro reconstitution of
PARP-1 interaction with chromatin, the development and testing of
small molecule PARP inhibitors, and the functions of understudied
members of PARP family. Written for the highly successful Methods
in Molecular Biology series, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Poly(ADP-Ribose) Polymerase: Methods
and Protocols, Second Edition serves as an ideal companion to the
first edition for scientists whose investigations involve this
important pathway. The chapter 'Identifying and Validating
Tankyrase Binders and Substrates: A Candidate Approach' is
published open access under a CC BY 4.0 license.
Poly (ADP-ribose) Polymerases (PARPs) are abundant and ubiquitous
proteins that regulate crucial processes of the cell cycle, DNA
repair, genomic stability, and transcriptional regulation. Being
involved in basic cell functions, PARPs mediate rapid responses to
such environmental factors as stress, infection, nutrition and
hormonal signals. Whereas PARP inhibitors can suppress tumor growth
and proliferation in certain breast, ovarian, and prostate cancers,
understanding how PARP controls cellular functions is essential for
the development of novel cancer treatments strategies. Divided into
three convenient sections, Poly(ADP-Ribose) Polymerase: Methods and
Protocols aims to explain how PARP proteins act within the normal
development of an organism as well as in pathogenic conditions,
seeks to advance the knowledge of developmental pathways
regulation, and endeavors to facilitate the development of new
therapeutic drugs and methods to target PARP-dependent processes.
Written in the highly successful Methods in Molecular Biology (TM)
series format, chapters contain introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Authoritative and
accessible, Poly(ADP-Ribose) Polymerase: Methods and Protocols
serves as an ideal guide to scientists who wish to continue
exploring this exciting and progressive research field.
Poly (ADP-ribose) Polymerases (PARPs) are abundant and ubiquitous
proteins that regulate crucial processes of the cell cycle, DNA
repair, genomic stability, and transcriptional regulation. Being
involved in basic cell functions, PARPs mediate rapid responses to
such environmental factors as stress, infection, nutrition and
hormonal signals. Whereas PARP inhibitors can suppress tumor growth
and proliferation in certain breast, ovarian, and prostate cancers,
understanding how PARP controls cellular functions is essential for
the development of novel cancer treatments strategies. Divided into
three convenient sections, Poly(ADP-Ribose) Polymerase: Methods and
Protocols aims to explain how PARP proteins act within the normal
development of an organism as well as in pathogenic conditions,
seeks to advance the knowledge of developmental pathways
regulation, and endeavors to facilitate the development of new
therapeutic drugs and methods to target PARP-dependent processes.
Written in the highly successful Methods in Molecular Biology (TM)
series format, chapters contain introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Authoritative and
accessible, Poly(ADP-Ribose) Polymerase: Methods and Protocols
serves as an ideal guide to scientists who wish to continue
exploring this exciting and progressive research field.
This detailed volume explores poly(ADP-ribose) polymerases (PARPs)
in the biology of eukaryotes and their relevance to human health.
Beginning with a section on the detection and quantification of
poly(ADP-ribose) polymer (pADPr), the book continues by delving
into the identification of protein targets, functional analysis,
the poly(ADP-ribosyl)ating pathway in chromatin and genes
expression, as well as the use of animal models and PARP1 inhibitor
design and testing, and more. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions
to their respective topics, lists of the necessary materials and
reagents, step-by-step and readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Poly(ADP-Ribose) Polymerase: Methods
and Protocols, Third Edition presents essential new and classical
methods for studying the pADPr-pathway.
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