This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression.
Key Features
* Provides well-tested experimental protocols for each technique
* Lists the reagents and preparation of each experiment separately
* Contains a complete schedule of experiments and the preparation required
* Includes study questions at the end of each chapter

This book helps understand Plato's writings by describing the circumstances in which they were produced. The author begins with an account of Plato's life and development and a brief analysis of some of the more difficult points arising from the criticism of Plato's writings. The remainder of the work considers the total setting - political, literary and philosophical - in which Plato's writings were produced. There are extensive appendices on the Platonic Epistles, Aristotle and the Theory of Ideas, and on the post-Aristotelian tradition. The result is both a lucid account of Plato himself and a comprehensive view of culture in fifth century Greece.

Blood, Sweat, and Toil is the first scholarly history of the British working class in the Second World War. It integrates social, political, and labour history, and reflects the most recent scholarship and debates on social class, gender, and the forging of identities. Geoffrey G. Field examines the war's impact on workers in the varied contexts of the family, military service, the workplace, local communities, and the nation. Previous studies of the Home Front have analysed the lives of civilians, but they have neglected the importance of social class in defining popular experience and its centrality in public attitudes, official policy, and the politics of the war years. Contrary to accounts that view the war as eroding class divisions and creating a new sense of social unity in Britain, Field argues that the 1940s was a crucial decade in which the deeply fragmented working class of the interwar decades was "remade," achieving new collective status, power, and solidarity. He criticizes recent revisionist scholarship that has downplayed the significance of class in British society. Extensively researched, using official documents, diaries and letters, the records of trade unions, and numerous other institutions, Blood, Sweat, and Toil traces the rapid growth of trade unionism, joint consultation, and strike actions in the war years. It also analyses the mobilization of women into factories and the uniformed services and the lives of men conscripted into the army, showing how these experiences shaped their social attitudes and aspirations. Using opinion polls and other evidence, Field traces the evolution of popular political attitudes from the evacuation of 1939 and the desperate months of late 1940 to the election of 1945, opposing recent claims that the electorate was indifferent or apathetic at the war's end but also eschewing blanket assumptions about popular radicalization. Labour was an active agent in fashioning itself as both a national progressive party and the representative of working-class interests in 1945; far from a mere passive beneficiary of anti-Tory feeling, it gave organizational form to the idealism and the demand for significant change that the war had generated.

Blood, Sweat, and Toil is the first scholarly history of the British working class in the Second World War. It integrates social, political, and labour history, and reflects the most recent scholarship and debates on social class, gender, and the forging of identities. Geoffrey Field examines the war's impact on workers in the varied contexts of the family, military service, the workplace, local communities, and the nation. Extensively researched, using official documents, diaries and letters, the records of trade unions and numerous other institutions, Blood, Sweat, and Toil traces the rapid growth of trade unionism, joint consultation, and strike actions in the war years. It also analyses the mobilization of women into factories and the uniformed services and the lives of men conscripted into the army, showing how these experiences shaped their aspirations and their social and political attitudes. Previous studies of the Home Front have analysed the lives of civilians, but they have neglected the importance of social class in defining popular experience and its centrality in public attitudes, official policy, and the politics of the war years. Contrary to accounts that view the war as eroding class divisions and creating a new sense of social unity in Britain, Field argues that the 1940s was a crucial decade in which the deeply fragmented working class of the interwar decades was 'remade', achieving new collective status, power, and solidarity. Employing a contingent, non-teleological conception of class identity and indicating the plural and shifting mix of factors that contributed to workers' social consciousness, he criticizes recent revisionist scholarship that has downplayed the significance of class in British society.

Japan's current shift from a manufacturing to a consumer economy is creating unprecedented opportunities for any company with the savvy to exploit this, the world's second largest market. Certainly, as the Japanese economy continues to rebound, more and more companies will continue to stake and build their presence there and use it as a springboard to enter other growing Asian markets. In Leveraging Japan, three leading authorities on market strategy and Japan present the new rules of Japanese marketing and discuss the evolution of other emerging Asian markets. These experts then share the same strategies that they've used to help American Express, Avon, Levi Strauss, and KFC, among other multinational companies, successfully establish a presence in Japan and leverage that presence to enter other Asian markets.
To read the first chapter from this book, click here.

The police, their methods, and their relations with the community had been the focus of considerable criticism and debate in the 1980s. While there were few books available on police training, it was widely recognised that training lay at the heart of many initiatives for police reform. Originally published in 1988, this book, based on a five-year study, provides a detailed picture of the training of police recruits in Britain at the time. The results themselves have centre stage in the book, which addresses questions basic to any working group: what kind of people join; whether they think the job changes them; their evaluation of the training and their officers; their thoughts on new policies such ass equal opportunities and community policing. A direct connection is made between the recruits’ impressions and experiences and their growing conception of what makes for good policing. The book traces how their attitudes to the force and their own roles change as they become familiar with the work and the occupational culture. The formal and informal socialization process is a crucial influence on the standards of competence which lie behind every contact between police and public and is central to our understanding of how the police operate. The author is a well-known researcher in the police world both here and in the USA. He relates his findings throughout to the North American experience, which provides valuable points of comparison. The important new material he presents informs debate and will still be of keen interest to students and researchers in the field.

Global environmental change (including climate change, biodiversity loss, changes in hydrological and biogeochemical cycles, and intensive exploitation of natural resources) is having significant impacts on the world's oceans. This handbook advances knowledge of the structure and functioning of marine ecosystems, and their past, present, and future responses to physical and anthropogenic forcing. It illustrates how climate and humans impact marine ecosystems, providing a comprehensive review of the physical and ecological processes that structure marine ecosystems as well as the observation, experimentation, and modelling approaches required for their study. Recognizing the interactive roles played by humans in using marine resources and in responding to global changes in marine systems, the book includes chapters on the human dimensions of marine ecosystem changes and on effective management approaches in this era of rapid change. A final section reviews the state of the art in predicting the responses of marine ecosystems to future global change scenarios with the intention of informing both future research agendas and marine management policy.
Marine Ecosystems and Global Change provides a detailed synthesis of the work conducted under the auspices of the Global Ocean Ecosystems Dynamics (GLOBEC) program. This research spans two decades, and represents the largest, multi-disciplinary, international effort focused on understanding the impacts of external forcing on the structure and dynamics of global marine ecosystems.

Global environmental change (including climate change, biodiversity loss, changes in hydrological and biogeochemical cycles, and intensive exploitation of natural resources) is having significant impacts on the world's oceans. This handbook advances knowledge of the structure and functioning of marine ecosystems, and their past, present, and future responses to physical and anthropogenic forcing. It illustrates how climate and humans impact marine ecosystems, providing a comprehensive review of the physical and ecological processes that structure marine ecosystems as well as the observation, experimentation, and modelling approaches required for their study. Recognizing the interactive roles played by humans in using marine resources and in responding to global changes in marine systems, the book includes chapters on the human dimensions of marine ecosystem changes and on effective management approaches in this era of rapid change. A final section reviews the state of the art in predicting the responses of marine ecosystems to future global change scenarios with the intention of informing both future research agendas and marine management policy.
Marine Ecosystems and Global Change provides a detailed synthesis of the work conducted under the auspices of the Global Ocean Ecosystems Dynamics (GLOBEC) program. This research spans two decades, and represents the largest, multi-disciplinary, international effort focused on understanding the impacts of external forcing on the structure and dynamics of global marine ecosystems.

Professionalizing the Police is a timely reassessment of the development of British police training and its contribution to the furtherance of the police professionalism agenda. The police have long struggled with the concept of professionalism. The Victorians veered from regarding police as servants to sanctifying policing as a special calling, while the supposed Golden Age of Policing was riven by divisions of class as sharp as those of the social diversity that poses one of contemporary policing's harshest tests. Police training has reflected these ambiguities and uncertainties. The ground its curriculum covers, pedagogy it employs, and structures through which it operates have been contested, troublesome to manage, and blamed for policing's failures. Behind these frictions lie large issues of governance, policing's place in society and what it means to be professional. Late modernity is marked by uncertainty and scepticism. In 'post-truth' times, professionalism must accommodate ambiguities of class, ethnicity and sexuality. The police languish as last believers in a monochrome vision of society while the norms that make for contemporary sociality have moved on to a multiplex of diversities that harbour new extremes both of tolerance and intolerance. True professionalism alerts practitioners to other ways of delivering social control and just societies: empowering citizens and encouraging autonomy; supporting new modes of social relationships and lifestyle; fitting provision to cases; pluralizing services. This yardstick is used to assess and challenge the recruit and in-service curriculum and to tease out the options around which professionalism can be configured and embedded such that it plays its part in a humane, coherent, and accountable framework of police governance. The book will appeal to academics and postgraduate students in police research (across criminology, sociology, psychology, socio-legal studies) and the professions (sociology, political science), as well as senior police managers and trainers in the police service and other applied government bodies.

A new turn in mixed methods research is here: merged methods. This provocative book offers a novel analysis of current mixed methods research, complicating traditional approaches and challenging existing techniques. Moving beyond the binary quantitative-qualitative distinction, the book presents methodologically grounded ways to merge methods in social research and integrate interpretive and structural approaches in one instrument or procedure. The book: Considers the importance of merging both epistemologies and methodologies. Showcases eight merged methods research approaches, from the Delphi method to multimodal content analysis. Explores the opportunities for merging methods using computational techniques, such as text mining. This innovative book is a must-read for any postgraduate student or researcher across the social sciences wanting to develop their understanding of mixed methods research.

Hell is different for everyone For Aron Atherton, hell is a small tropical island with white sand beaches surrounded by the clear blue waters of the Indian Ocean. Trapped there after a massive solar storm killed his family and nearly wiped out the human race, he plans to fish and drink his way through the apocalypse. But when his best friend is murdered, Aron's plan fizzles away like the vanishing skies above him. Now the guardian of a ten year old boy, Aron is drawn into a struggle that will decide who leaves the dying planet and who stays behind. And as he tries to escape the vortex of political corruption that engulfs him, he uncovers a plot that threatens mankind's last hope for survival. With an army of fanatical pirates about to launch an attack against the islands, Aron must stay alive long enough to expose the people behind the plot. For a man who'd rather be fishing, this is hell.

This scarce antiquarian book is included in our special Legacy Reprint Series. In the interest of creating a more extensive selection of rare historical book reprints, we have chosen to reproduce this title even though it may possibly have occasional imperfections such as missing and blurred pages, missing text, poor pictures, markings, dark backgrounds and other reproduction issues beyond our control. Because this work is culturally important, we have made it available as a part of our commitment to protecting, preserving and promoting the world's literature.

This scarce antiquarian book is included in our special Legacy Reprint Series. In the interest of creating a more extensive selection of rare historical book reprints, we have chosen to reproduce this title even though it may possibly have occasional imperfections such as missing and blurred pages, missing text, poor pictures, markings, dark backgrounds and other reproduction issues beyond our control. Because this work is culturally important, we have made it available as a part of our commitment to protecting, preserving and promoting the world's literature.

This is an EXACT reproduction of a book published before 1923. This IS NOT an OCR'd book with strange characters, introduced typographical errors, and jumbled words. This book may have occasional imperfections such as missing or blurred pages, poor pictures, errant marks, etc. that were either part of the original artifact, or were introduced by the scanning process. We believe this work is culturally important, and despite the imperfections, have elected to bring it back into print as part of our continuing commitment to the preservation of printed works worldwide. We appreciate your understanding of the imperfections in the preservation process, and hope you enjoy this valuable book.

This scarce antiquarian book is included in our special Legacy Reprint Series. In the interest of creating a more extensive selection of rare historical book reprints, we have chosen to reproduce this title even though it may possibly have occasional imperfections such as missing and blurred pages, missing text, poor pictures, markings, dark backgrounds and other reproduction issues beyond our control. Because this work is culturally important, we have made it available as a part of our commitment to protecting, preserving and promoting the world's literature.

This scarce antiquarian book is included in our special Legacy Reprint Series. In the interest of creating a more extensive selection of rare historical book reprints, we have chosen to reproduce this title even though it may possibly have occasional imperfections such as missing and blurred pages, missing text, poor pictures, markings, dark backgrounds and other reproduction issues beyond our control. Because this work is culturally important, we have made it available as a part of our commitment to protecting, preserving and promoting the world's literature.

A new turn in mixed methods research is here: merged methods. This provocative book offers a novel analysis of current mixed methods research, complicating traditional approaches and challenging existing techniques. Moving beyond the binary quantitative-qualitative distinction, the book presents methodologically grounded ways to merge methods in social research and integrate interpretive and structural approaches in one instrument or procedure. The book: Considers the importance of merging both epistemologies and methodologies. Showcases eight merged methods research approaches, from the Delphi method to multimodal content analysis. Explores the opportunities for merging methods using computational techniques, such as text mining. This innovative book is a must-read for any postgraduate student or researcher across the social sciences wanting to develop their understanding of mixed methods research.