Human beings have succeeded as the most dominant species on earth in large part due to our need to connect and cooperate. It was our ability to socialize and connect that catapulted our species to phenomenal heights of innovation, through collaboration and specialization. This drive has fine-tuned our unconscious perception of faces, facial expressions, body language, and touch. Our primitive drive to connect changes how we perceive the world and the people around us. We see, hear, empathize with, and understand others differently depending on whether they are a member of our in-group or not. This unconscious drive to connect can draw us together, but it also emphasizes the differences between groups. And it is getting worse, as overcrowding, technology, and the media often focus us on our differences. We become more and more divided into groups as a result. Here, Mark Williams shows us how to recapture the drive for connection in a way that will help us look past our differences and reconnect, even with those whom we perceive to be outside our groups. He starts by discussing the human brain's specialization for connection and how it evolved, and the fascinating way we automatically process the thoughts and feelings of others. He focuses on how connection works in practice and why it is important for learning, innovating, health and wellbeing. He then explores the negative consequences of our drive for connection and explains how it contributes to racism, sexism, nationalism, and many other social issues of our day, as well as its impact on our individual health and wellbeing. He ends with a positive perspective by examining how we can use our drive for connection to expand our in-group and extend multicultural societies for the good of our planet.

This third edition provides new and updated chapters detailing a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins with their natural ligands, drugs or other binding partners. Chapters detail natural substrates, potential drug leads, quantitative understanding of the mechanism of interaction, and different techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Third Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions.

Proteins are the cell's workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions - whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology.

Proteins are the cell's workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions - whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology.

*Exploits new features of Visual Studio .NET 2003 and the Oracle database 10g. (But concepts and code still apply to the earlier releases, 8i and 9i, that currently comprise most business systems). *Explains Oracle programming using both C# and VB.NET languages; provides a one-stop reference for VS.NET developers working with Oracle. *Excellent resource for J2EE programmers in environments considering moving to a .NET environment (and retaining the Oracle database).

This third edition provides new and updated chapters detailing a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins with their natural ligands, drugs or other binding partners. Chapters detail natural substrates, potential drug leads, quantitative understanding of the mechanism of interaction, and different techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Third Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions.

Light the Wings, Remove the Non-Existent Speed is a book of poetry that pushes against the everyday ordinary. It encourages us to look out for all this world has to offer, drop what makes us slower, and find the lights that certainly help us see a little more.

Light the Wings, Remove the Non-Existent Speed is a book of poetry that pushes against the everyday ordinary. It encourages us to look out for all this world has to offer, drop what makes us slower, and find the lights that certainly help us see a little more.