This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.

Prenatal life is the period of maximal development in animals, and it is well recognised that factors that alter development can have profound effects on the embryonic, fetal and postnatal animal. Scientists involved in research on livestock productivity have for decades studied postnatal consequences of fetal development on productivity. Recently, however, there has been a surge in interest in how to manage prenatal development to enhance livestock health and productivity. This has occurred largely due to the studies that show human health in later life can be influenced by events during prenatal life, and establishment of the Fetal Origins and the Thrifty Phenotype Hypotheses. This book, Managing the Prenatal Environment to Enhance Livestock Productivity reviews phenotypic consequences of prenatal development, and provides details of mechanisms that underpin these effects in ruminants, pigs and poultry. The chapters have been divided into three parts: Quantification of prenatal effects on postnatal productivity, mechanistic bases of postnatal consequences of prenatal development and regulators of fetal and neonatal nutrient supply. Managing the Prenatal Environment to Enhance Livestock Productivity is a reference from which future research to improve the level of understanding and capacity to enhance productivity, health and efficiency of livestock in developing and developed countries will evolve. It is particularly timely given the development of molecular technologies that are providing new insight into regulation and consequences of growth and development of the embryo, fetus and neonate.

In this book internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors and Design and Characterization of Gene Transfer Vectors. Readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for applications in stem cell biology, cancer research and infectious disease.

The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The chapters are written by world-renowned experts and the volume's five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes.

For nearly 50 years, pest control was mostly based on broad-spectrum conv- tional insecticides such as organochlorines, organophosphates, carbamates and pyrethroids. However, the severe adverse effects of pesticides on the environment, problems of resistance reaching crisis proportions and public protests led to stricter regulations and legislation aimed at reducing their use. Ways to reduce the use of synthetic pesticides in plant protection and to use more alternative and novel me- ods for pest control or biorational control are the challenges of pest control for the twenty-first century. The term biorational (biological + rational) pesticides can be defined as the use of specific and selective chemicals, often with a unique modes of action, that are compatible with natural enemies and the environment, with minimal effect on n- target organisms. Biorational control is based on a diversity of chemical, biological and physical approaches for controlling insect pests which results in minimum risk to man and the environment.

This book, written by experienced geneticists, covers topics ranging from the natural history of the mouse species, its handling and reproduction in the laboratory, and its classical genetics and cytogenetics, to modern issues including the analysis of the transcriptome, the parental imprinting and X-chromosome inactivation. The strategies for creating all sorts of mutations, either by genetic engineering or by using mutagens, are also reviewed and discussed in detail. Finally, a last chapter outlines the methodology used for the analysis of complex or quantitative traits. The authors also discuss the importance of accurate phenotyping, which is now performed in the mouse clinics established worldwide and identify the limits of the mouse model, which under certain circumstances can fail to present the phenotype expected from the cognate condition in the human model. For each chapter an up-to-date list of pertinent references is provided. In short, this book offers an essential resource for all scientists who use or plan to use mice in their research.

Written by an international panel of recognized leaders in the field, Neural Crest Induction and Differentiation discusses all aspects of modern neural crest biology from its evolutionary significance to its specification, migration, plasticity and contribution to multiple lineages of the vertebrate body, to the pathologies associated with abnormal neural crest development and function. Abundant color figures enhance the text providing clear and attractive illustrations of central issues and concepts.

The annual Evolutionary Biology Meetings in Marseilles serve to gather leading evolutionary biologists and other scientists using evolutionary biology concepts, e.g. for medical research. The aims of these meetings are to promote the exchange of ideas and to encourage interdisciplinary collaborations. This book collects 19 selected contributions presented at the 15th meeting, which took place in September 2011. It starts with a description of the life and work of J.B.S. Haldane, a remarkable evolutionary biologist of the 20th century. The remaining chapters are grouped under the following three themes: * New Concepts in Evolutionary Biology * Macroevolution: Mechanisms and Trends * Genome Evolution Offering an up-to-date overview of recent results in the field of evolutionary biology, this book is an invaluable source of information for scientists, teachers and advanced students.

The annual Evolutionary Biology Meetings in Marseille aim to bring together leading scientists, promoting an exchange of state-of-the-art knowledge and the formation of inter-group collaborations. This book presents the most representative contributions to the 13th meeting, which was held in September 2009. It comprises 21 chapters, which are organized into the following three categories: * Evolutionary Biology Concepts * Genome/Molecular Evolution * Morphological Evolution/Speciation This book offers an up-to-date overview of evolutionary biology concepts and their use in the biology of the 21st century.

In light of mounting fishing pressures, increased aquaculture production and a growing concern for fish well-being, improved knowledge on the swimming physiology of fish and its application to fisheries science and aquaculture is needed. This book presents recent investigations into some of the most extreme examples of swimming migrations in salmons, eels and tunas, integrating knowledge on their performance in the laboratory with that in their natural environment. For the first time, the application of swimming in aquaculture is explored by assessing the potential impacts and beneficial effects. The modified nutritional requirements of "athletic" fish are reviewed as well as the effects of exercise on muscle composition and meat quality using state-of-the-art techniques in genomics and proteomics. The last chapters introduce zebrafish as a novel exercise model and present the latest technologies for studying fish swimming and aquaculture applications.

In this book, Dr. Li and his author team plan to emphasize why mouse models are useful in vivo systems for understanding disease mechanisms and developing therapeutic strategies in blood cancers. The authors do not intend to cover all types of blood cancers; instead, they will focus on some major ones such as leukemias and lymphomas. However, the authors will try to cover as much as they can the cancer types and point out that many blood cancers need to be studied in mouse disease models although they are still not available at present. A major focus in the book will be to show what we can or cannot learn from mouse disease models and to also show the critical contributions of mouse models in therapeutic drug development.

The ontogeny of each individual contributes to the physical, physiological, cognitive, neurobiological, and behavioral capacity to manage the complex social relationships and diverse foraging tasks that characterize the primate order. For these reasons Building Babies explores the dynamic multigenerational processes of primate development. The book is organized thematically along the developmental trajectory:conception, pregnancy, lactation, the mother-infant dyad, broader social relationships, and transitions to independence. In this volume, the authors showcase the myriad approaches to understanding primate developmental trajectories from both proximate and ultimate perspectives. These collected chapters provide insights from experimental manipulations in captive settings to long-term observations of wild-living populations and consider levels of analysis from molecule to organism to social group to taxon. Strepsirrhines, New World monkeys, Old World monkeys, apes, and humans are all well-represented. Contributions by anthropologists, microbiologists, psychologists, population geneticists, and other primate experts provide Building Babies a uniquely diverse voice. Building Babies features multi- and trans-disciplinary research approaches to primate developmental trajectories and is particularly useful for researchers and instructors in anthropology, animal behavior, psychology, and evolutionary biology. This book also serves as a supplement to upper-level undergraduate courses or graduate seminars on primate life history and development. In these contexts, the book provides exposure to a wide range of methodological and theoretical perspectives on developmental trajectories and models how researchers might productively integrate such approaches into their own work.

Bemisia tabaci (Gennedius) has distinguished itself from the more than 1,000 whitefly species in the world by its adaptability, persistence and potential to damage a wide range of agricultural and horticultural crops in all six of the world's inhabited continents. B. tabaci inflicts plant damage through direct feeding, inducement of plant disorders, vectoring of plant viruses and excretion of honeydew. This book collates multiple aspects of the pest ranging from basic to applied science and molecular to landscape levels of investigation. Experts in multiple disciplines provide broad, but detailed summaries and discussion of taxonomy, genetics, anatomy, morphology, physiology, behavior, ecology, symbiotic relationships, virus vector associations and various tactics for integrated management of this pest insect. The book is focused primarily on progress during the last 10-15 years and is directed at workers in the field as well as the informed professional who may not necessarily specialize in whitefly research. The book is unique in providing broad coverage in relatively few chapters by recognized experts that highlight the state-of-the-art in our understanding of this fascinating but troublesome cosmopolitan pest.

Cell culture based research is important for our understanding of biological processes at the cellular and molecular level. Using this approach, the previous decades have produced a wealth of mechanistic information in all areas of biomedical research. Such in vitro research, however, lacks the complexity of in vivo investigations, where many different cell types interact with each other in a normal, three-dimensional environment, with normal levels of cytokines and growth factors. Furthermore, complex human diseases, such as cancer, diabetes or chronic inflammation, can only be modeled in vivo. Due to its small size, its short reproduction time, and the possibility to introduce specific gene mutations, the mouse has become the favourite mammalian model organism to study in vivo function of genes during development and in disease. This book combines review articles on selected subjects presented at the symposium "Mouse as a Model Organism - From Animals to Cells", held in Rovaniemi, Finland, 2009. Among other topics, high-throughput phenotyping of mouse mutants, mouse phenotypes dependent on nature and nuture, and a spectrum of in vivo, ex vivo and in vitro methods to study cancer in mice are described. This book will give an excellent introduction to scientists interested in the use of mice as a model to understand complex biological questions in the post-genomic era. It will highlight the possibilities, but also discuss the current problems and shortcomings, to give a realistic view of the current state-of-art in this fascinating field of biomedical research.

Marsupials belong to the Class Mammalia, sharing some features with other mammals, yet they also possess many unique features. It is their differences from the more traditionally studied mammals, such as mice and humans, that is of greatest value to comparative studies. Sequencing of genomes from two distantly related marsupials, the short grey-tailed opossum from South America and the Australian tammar wallaby, has launched marsupials into the genomics era and accelerated the rate of progress in marsupial research. With the current worldwide concern for the plight of the endangered Tasmanian devil, marsupial genetics and genomics research is even more important than ever if this species is to be saved from extinction. This volume recounts some of the history of research in this field and highlights the most recent advances in the many different areas of marsupial genetics and genomics research.

The evolution of leks--clusters of small territories where males congregate and display in order to attract mates--is of central issue in behavioral ecology, because of the insights it offers into female mate choice, sexual selection, and the evolution of mating systems. In the first book on the subject, Jacob HA

glund and Rauno Alatalo draw together existing knowledge on two main aspects of lekking. Why do leks evolve in some species and not in others? Why do females of certain lekking species select their mates even though such behavior reaps few or no material benefits for them? In each case they emphasize the importance of understanding the selective forces that act on individuals in natural populations.

HA

glund and Alatalo synthesize the available information on lekking in all animal groups and suggest new areas of research.

Originally published in 1995.

The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These paperback editions preserve the original texts of these important books while presenting them in durable paperback editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905."

"Mouse Genetics: Methods and Protocols" provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish" "the manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly successful "Methods in Molecular Biology "series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.

Comprehensive and authoritative, "Mouse Genetics: Methods and Protocols" promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.

The Second Georgia Genetics Symposium was held color. Soon after, he joined the sta? of The Jackson in September 2000, and the development of this Laboratory in Bar Harbor, Maine. book took place over the nearly 4 years that ensued. Much of Bill's research at the lab was centered During this time, many advances in the Genome around investigating phenotypic variability within Project and mouse mutagenesis were made. In the highly inbred strains, and in that connection he book overview, we discuss the development of the developed the technique of ovarian transplanta- Genome Project (which is the context for the sym- tion (even using embryonic donors) and a genetic posium), the role the mouse was playing at that scheme whereby graft compatibility could be time, how that role has evolved, and how the combined with the ability to distinguish o?spring chapters of the book address issues in mouse func- from donor and regenerated host ovaries. His tional genetics. Many of the chapters in this book work was in?uenced by the second World War, will provide useful resources for years to come. ?rst because The Jackson Laboratory turned into Of greater impact, our keynote speaker, the a production colony for the military, primarily to mutagenesis pioneer William L. (Bill) Russell, produce mice for typhoid testing, and secondly, passed away on July 23, 2003.

Foreword by Phillip V. Tobias The introduction of rhesus macaques to Cayo Santiago, Puerto Rico in 1938, and the subsequent development of the CPRC for biomedical research, continues its long history of stimulating studies in physical anthropology. The CPRC monkey colonies, and the precise demographic data on the derived skeletal collection in the Center's Laboratory of Primate Morphology and Genetics (LPMG), provide rare opportunities for morphological, developmental, functional, genetic, and behavioral studies across the life span of rhesus macaques as a species, and as a primate model for humans. The book grows out of a symposium Wang is organizing for the 78th annual meeting of the American Association of Physical Anthropologists to be held in April 2009. This symposium will highlight recent and ongoing research in, or related to, physical anthropology, and reveal the numerous research opportunities that still exist at this unusual rhesus facility. Following an initial historical review of CPRC and its research activities, this book will emphasize recent and current researches on growth, function, genetics, pathology, aging, and behavior, and the impact of these researches on our understanding of rhesus and human morphology, development, genetics, and behavior. Fourteen researchers will present recent and current studies on morphology, genetics, and behavior, with relevance to primate and human growth, health, and evolution. The book will include not only papers presented in the symposium, but also papers from individuals who could not present their work at the meeting due to limitations in the maximum number (14) of permitted speakers.

In Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study mouse embryology. These include protocols and techniques that are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA. With additional chapters on fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research.

This volume provides a comprehensive selection of recent studies addressing insect hearing and acoustic communication. The variety of signalling behaviours and hearing organs makes insects highly suitable animals for exploring and analysing signal generation and hearing in the context of neural processing, ecology, evolution and genetics. Across a variety of hearing species like moths, crickets, bush-crickets, grasshoppers, cicadas and flies, the leading researchers in the field cover recent scientific progress and address key points in current research, such as:

. How can we approach the evolution of hearing in insects and what is the developmental and neural origin of the auditory organs?

. How are hearing and sound production embedded in the natural lifestyle of the animals, allowing intraspecific communication but also predator avoidance and even predation?

. What are the functional properties of hearing organs and how are they achieved at the molecular, biophysical and neural levels?

. What are the neural mechanisms of central auditory processing and signal generation?

The book is intended for students and researchers both inside and outside of the fascinating field of bioacoustics and aims to foster understanding of hearing and acoustic communication in insects."

This book provides an evolutionary conceptual framework for comparative genomics, with the ultimate objective of understanding the loss and gain of genes during evolution, the interactions among gene products, and the relationship between genotype, phenotype and the environment. The many examples in the book have been carefully chosen from primary research literature based on two criteria: their biological insight and their pedagogical merit. The phylogeny-based comparative methods, involving both continuous and discrete variables, often represent a stumbling block for many students entering the field of comparative genomics. They are numerically illustrated and explained in great detail. The book is intended for researchers new to the field, i.e., advanced undergraduate students, postgraduates and postdoctoral fellows, although professional researchers who are not in the area of comparative genomics will also find the book informative.

The order Rodentia is the most abundant and successful group of mammals, and it has been a focal point of attention for compar ative and evolutionary biologists for many years. In addition, rodents are the most commonly used experimental mammals for bio medical research, and they have played a central role in investi gations of the genetic and molecular mechanisms of speciation in mammals. During recent decades, a tremendous amount of new data from various aspects of the biology of living and fossil rodents has been accumulated by specialists from different disciplines, ranging from molecular biology to paleontology. Paradoxically, our understanding of the possible evolutionary relationships among different rodent families, as well as the possible affinities of rodents with other eutherian mammals, has not kept pace with this information "explosion. " This abundance of new biological data has not been incorporated into a broad synthesis of rodent phylo geny, in part because of the difficulty for any single student of rodent evolution to evaluate the phylogenetic significance of new findings from such diverse disciplines as paleontology, embryology, comparative anatomy, molecular biology, and cytogenetics. The origin and subsequent radiation of the order Rodentia were based primarily on the acquisition of a key character complex: specializations of the incisors, cheek teeth, and associated mus culoskeletal features of the jaws and skull for gnawing and chewing."

Kisspeptin has been shown to be both necessary and sufficient for activation of the reproductive axis, during puberty and later in adulthood. This makes kisspeptin a fundamental component of the reproductive axis. Kisspeptin has been deemed the single most potent stimulator of GnRH neurons yet known. The importance of kisspeptin has been documented in humans as well as non-human animal models, ranging from monkeys, sheep, and rodents to numerous fish species, thus signifying a highly conserved nature of its reproductive function. Importantly, kisspeptin neurons seem to mediate many of the regulatory effects of other signals, whether they are metabolic, circadian, hormonal, or stress. This places kisspeptin neurons in a unique position to be key nodal points and conduits for conveying numerous endogenous and exogenous signals to the reproductive axis.