More than two third of the surface area of our planet is covered by oceans and assessment of the marine biodiversity is a challenging task. With the increasing global population, there is a tendency to exploit marine recourses for food, energy and other requirements. This puts pressure on the fragile marine environment and needs sustainable conservation efforts. Marine species identification using traditional taxonomical methods are often burdened with taxonomic controversies. Here in this book we will discuss the comparatively new concept of DNA barcoding and its significance in marine perspective. This molecular technique can be helpful in the assessment of cryptic species which are widespread in marine environment, and can also be used to link the different life cycle stages to the adult which is difficult to accomplish in marine ecosystems. Other advantages of DNA barcoding include authentication and safety assessment of seafood, wildlife forensics, conservation genetics and detection of invasive alien species (IAS). Global DNA barcoding efforts in the marine habitat include MarBOL, CeDAMar, CMarZ, SHARK-BOL, etc. DNA barcoding of different marine groups ranging from the microbes to mammals is to be revealed. In conjugation with newer and faster techniques like high throughput sequencing, DNA barcoding is serving as an effective modern tool in marine biodiversity assessment and conservation.

Bemisia tabaci (Gennedius) has distinguished itself from the more than 1,000 whitefly species in the world by its adaptability, persistence and potential to damage a wide range of agricultural and horticultural crops in all six of the world s inhabited continents. B. tabaci inflicts plant damage through direct feeding, inducement of plant disorders, vectoring of plant viruses and excretion of honeydew. This book collates multiple aspects of the pest ranging from basic to applied science and molecular to landscape levels of investigation. Experts in multiple disciplines provide broad, but detailed summaries and discussion of taxonomy, genetics, anatomy, morphology, physiology, behavior, ecology, symbiotic relationships, virus vector associations and various tactics for integrated management of this pest insect. The book is focused primarily on progress during the last 10-15 years and is directed at workers in the field as well as the informed professional who may not necessarily specialize in whitefly research. The book is unique in providing broad coverage in relatively few chapters by recognized experts that highlight the state-of-the-art in our understanding of this fascinating but troublesome cosmopolitan pest."

Gonadotropin-releasing hormone (GnRH) cells are the key regulators of reproductive function in all vertebrate organisms. The GnRH molecule is synthesized in a small number of neurons in rostral hypothalamic regions of the brain. In mammals, these neurons release the GnRH decapeptide into the portal capillary system leading to the anterior pituitary gland. There, GnRH causes the release of the gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), which in turn act upon the gonads to stimulate their maturation, and to cause synthesis of sex steroid hormones, estrogen, progesterone and testosterone. Although each of the levels of the hypothalamic-pituitary-gonadal axis is critical for reproductive function, GnRH neurons play the primary role in the control of reproductive maturation and adult reproductive function, and may even play a role in reproductive senescence.

Since its discovery in 1970, there has been intense interest in GnRH-producing neurons, with more than 8000 papers and chapters in the last decade alone. Despite this activity of research in basic and clinical science, there has never been a book written specifically on GnRH neurons. GnRH: The Master Molecule of Reproduction aims to bring together the large and diverse literature of both laboratory and applied research that focuses on these unique cells.

This book will provide basic background into reproductive neuroendocrinology, as well as specifics regarding the role of GnRH neurons in the control of reproduction. Students studying endocrinology, reproduction, neuroendocrinology or molecular endocrinology will benefit from this book. In addition, this book will take a multi-species approach which will be useful both to basic researchers as well as clinicians. Whenever possible, species differences and similarities will be presented, and if possible, studies on humans, or the clinical relevance of basic research findings to humans will be discussed (such as the treatment of reproductive disorders such as abnormalities in pubertal development, or infertility).

Metamorphosis addresses various facets of postembryonic development, particularly signal transduction, morphogenesis, cell-cell interactions, and programmed cell death. A key feature of the book is its exploration of the molecular mechanisms underlying these processes.
Key Features
* Hormonal regulation of development
* The mechanisms of hormone action
* The steroid/thyroid hormone receptor family
* Morphogenesis and programmed cell death

Cell culture based research is important for our understanding of biological processes at the cellular and molecular level. Using this approach, the previous decades have produced a wealth of mechanistic information in all areas of biomedical research. Such in vitro research, however, lacks the complexity of in vivo investigations, where many different cell types interact with each other in a normal, three-dimensional environment, with normal levels of cytokines and growth factors. Furthermore, complex human diseases, such as cancer, diabetes or chronic inflammation, can only be modeled in vivo. Due to its small size, its short reproduction time, and the possibility to introduce specific gene mutations, the mouse has become the favourite mammalian model organism to study in vivo function of genes during development and in disease. This book combines review articles on selected subjects presented at the symposium "Mouse as a Model Organism - From Animals to Cells", held in Rovaniemi, Finland, 2009. Among other topics, high-throughput phenotyping of mouse mutants, mouse phenotypes dependent on nature and nuture, and a spectrum of in vivo, ex vivo and in vitro methods to study cancer in mice are described. This book will give an excellent introduction to scientists interested in the use of mice as a model to understand complex biological questions in the post-genomic era. It will highlight the possibilities, but also discuss the current problems and shortcomings, to give a realistic view of the current state-of-art in this fascinating field of biomedical research.

Entomology as a science of inter-depended branches like molecular entomology, insect biotechnology, has made rapid progress. This also implies that there is an urgent need to manage the available resources. In the past five decades, entomology has taken giant steps ahead. The aim of this work is to integrate perspectives across molecular and biochemistry, physiology, reproduction, developmental biology, molecular evolution, genetics and RNAi applications. This century is proclaimed as the Era of Biotechnology and it consists of all types of Mol-Bio-Gen applications, which is an essential component for a thorough understanding of the insect biology. The aim of this work is to provide the comprehensive review of recent research from various geographic areas around the world and contributing authors that are recognized experts in their respective field of Genomic entomology. This Volume emphasizes upon the need for and relevance of studying molecular aspects of entomology in Universities, Agricultural Universities and other centers of molecular research. It will also serve as a landmark source for Insect advance science technology.

One of the mysteries of mammalian reproduction is the physiologic process that determines the length of gestation. The proper timing of birth ensures that the young individual is sufficiently developed to survive and adapt in the extrauterine environment, and that the mother is capable to provide nutrition and protection to the newborn. This volume summarizes new knowledge obtained by many researchers seeking to unravel the compile mechanisms that contribute to the maintenance and termination of pregnancy. The most important common goal of these efforts is to reduce the incidence of preterm birth, which is the leading cause of perinatal morbidity and mortality in numerous countries.
Separate chapters are devoted to the best-studied animal models of parturition. In sheep, the fetus is in control of the timing of its own birth, while in avian species, oviposition is evidently determined by the female laying the feritlized egg. In humans and non-human primates, the roles of the fetus and the mother are more balanced, and involve a complicated and poorly understood interplay between the mother, the fetus, and the placenta. Some major aspects of these interactions, such as trophoblast function, myometrial contractility, and the endocrine-paracrine systems, are discussed in further chapters.

Every year there are new and exciting developments in assisted human reproduction, but how much do we really know about the underlying causes of infertility? This volume explores recent progress in the understanding of the genetics of spermatogenesis and male infertility. Topics include fundamental advances and current problems in the development and function of the testis, an outline of clinical findings in male infertility and an overview of the role of the Y chromosome in male fertility. Comprehensive critiques of posttranscriptional control during spermatogenesis, mammalian meiotic sterility, and comparative genetics of human spermatogenesis from the perspective of yeast, "Drosophila" and mice provide a global overview of the field.

The annual Evolutionary Biology Meetings in Marseille aim to bring together leading scientists, promoting an exchange of state-of-the-art knowledge and the formation of inter-group collaborations. This book presents the most representative contributions to the 13th meeting, which was held in September 2009. It comprises 21 chapters, which are organized into the following three categories: * Evolutionary Biology Concepts * Genome/Molecular Evolution * Morphological Evolution/Speciation This book offers an up-to-date overview of evolutionary biology concepts and their use in the biology of the 21st century.

Upcoming applications of genetic engineering in farm animals include higher yields, leaner meat, or disease resistance. The proceedings cover an analysis of the state of the art of the technology and its applications, an introduction to the specific application zoopharming (a method to produce biopharmaceuticals in transgenic livestock), including an analysis of the market for biopharmaceuticals. In addition an assessment of ethical aspects of livestock biotechnology and considerations regarding animal welfare implications are covered. The study is addressed to science, industry and politics.

This book presents selected contributions to the 19th Evolutionary Biology Meeting, which took place in September 2015 in Marseille. It consists of 22 chapters, which are grouped in four sections: * Convergent Evolution * Evolution of Complex Traits * Concepts * Methods The annual Evolutionary Biology Meetings in Marseille serve to gather leading evolutionary biologists and other scientists using evolutionary biology concepts, e.g for medical research, to promote the exchange of ideas and to encourage interdisciplinary collaborations. Offering an up-to-date overview of recent findings in the field of evolutionary biology, this book is an invaluable source of information for scientists, teachers and advanced students.

This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.

This volume includes twelve novel empirical papers focusing on the behaviour and cognition of both captive and wild bonobos (Pan paniscus). As our species less known closest relative, the bonobo has gone from being little studied to increasingly popular as a species of focus over the past decade. Overall this volume demonstrates how anyone interested in understanding humans or chimpanzees must also know bonobos. Bonobos are not only equal to chimpanzees as our relatives, but they are also unique. The majority of papers in this volume show that whether you are interested in the evolution of culture and tool use, social relationships and sharing or foraging ecology and cognition, bonobos have a major contribution to make. Four papers provide further evidence that the behaviour and psychology of bonobo females is radically different from that observed in chimpanzees. Foraging behaviour and cognition of bonobos is the focus of three papers that each show important ways that bonobos spatial cognition differs remarkably from chimpanzees. Two papers are relevant to solving the puzzle of why bonobos are expert extractive foragers in captivity but have never been seen using tools to obtain food in the wild. The articles presented in this volume are previously published in a Special Issue of Behaviour, Volume 152, Parts 3-4 (March 2015).

In Molecular Embryology: Methods and Protocols, Second Edition, expert investigators provide a comprehensive guide to the cutting-edge methods used across the dramatically growing field of vertebrate molecular embryology. Time-tested techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The second edition collects classic protocols which have become standard techniques in the laboratory and presents them in a complementary fashion with novel and emerging approaches, allowing researcher to become more familiar with commonly studied embryos used in biomedical research. Insightful to the experienced professional, Molecular Embryology: Methods and Protocols, Second Edition, presents cutting-edge findings of perhaps the greatest period in growth and productivity in the field of developmental biology.

Newborn mammals can weigh as little as a dime or as much as a motorcycle. Some receive milk for only a few days, whereas others nurse for years. Humans typically have only one baby at a time following nine months of pregnancy, but other mammals have twenty or more young after only a few weeks in utero. What causes this incredible reproductive diversity? In Reproduction in Mammals, Virginia Hayssen and Teri Orr present readers with a fascinating examination of the varied reproductive strategies of a broad spectrum of mammals, from marsupials to whales. This unique book's comprehensive coverage gathers stories from many taxa into a single, cohesive perspective that centers on the reproductive lives of females. The authors shed light on a number of intriguing questions, including * do bigger moms have bigger babies?* do primates have longer pregnancies than other groups?* does habitat influence animals' reproductive patterns?* do carnivores typically produce larger litters than prey species? The book opens with the authors' definition of what constitutes a female perspective and an examination of the evolution of reproduction in mammals. It then outlines the typical individual mammalian female: her genetics, anatomy, and physiology. Taking a nuanced approach, Hayssen and Orr describe the female reproductive cycle and explore female mammals' interactions with males and offspring. Readers will come away from this thought-provoking book with an understanding not only of how reproduction fits into the lives of female mammals but also of how biology has affected the enormously diverse reproductive patterns of the phenotypes we observe today.

Comparative Vertebrate Reproduction is the only comprehensive textbook covering major topics in the reproductive biology of vertebrates, from sexuality and gametogenesis to reproductive ecology and life history tactics. The work draws heavily on recent reviews and papers while placing topics in a historical context and conceptual framework. In addition, the author provides detailed comparative surveys of each of the major topics discussed. Comparative Vertebrate Reproduction has been written as a textbook for upper-level undergraduate and graduate-level students in biology, zoology, physiology, animal science, and veterinary medicine. The work also serves as an excellent reference for researchers in medical and veterinary schools working in reproductive medicine.

"Mouse Genetics: Methods and Protocols" provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish" "the manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly successful "Methods in Molecular Biology "series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.

Comprehensive and authoritative, "Mouse Genetics: Methods and Protocols" promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.

Many genes have been cloned from chicken cells, and during the next decade numerous laboratories will be concentrating their resources in developing ways of using these tools. Manipulation of the Avian Genome contains the most recent information from leading research laboratories in the areas of developmental and molecular genetics of the chicken. This information was presented at the Keystone Symposium held at Lake Tahoe in March, 1991. The book discusses potential applications of emerging technology in basic science and poultry production. Various techniques for altering genomic DNA, such as microinjection, retroviral vectors, and lipofection are covered. Genome evaluation using DNA fingerprinting and conventional breeding techniques are presented.

The overall scope of this new series will be to evolve an understanding of the genetic basis of (1) how early mesoderm commits to cells of a heart lineage that progressively and irreversibly assemble into a segmented, primary heart tube that can be remodeled into a four-chambered organ, and (2) how blood vessels are derived and assembled both in the heart and in the body. Our central aim is to establish a four-dimensional, spatiotemporal foundation for the heart and blood vessels that can be genetically dissected for function and mechanism. Since Robert DeHaan's seminal chapter "Morphogenesis of the Vertebrate Heart" published in Organogenesis (Holt Rinehart & Winston, NY) in 1965, there have been surprisingly few books devoted to the subject of cardiovascular morpho genesis, despite the enormous growth of interest that occurred nationally and inter nationally. Most writings on the subject have been scholarly compilations of the proceedings of major national or international symposia or multi authored volumes, without a specific theme. What is missing are the unifying concepts that can often make sense out of a burgeoning database of facts. The Editorial Board of this new series believes the time has come for a book series dedicated to cardiovascular mor not only as an important archival and didactic reference phogenesis that will serve source for those who have recently come into the field but also as a guide to the evo lution of a field that is clearly coming of age.

One of the striking findings of modern developmental biology has been the high degree of conservation of signaling and developmental mechanisms amongst different animal species. Such conservation allows information learned from a given organism to be applicable to other species, including humans, and has validated the use of a few model systems to deduce general biological principles. In spite of this underlying conservation, however, each species has unique characteristics arising from its evolutionary history. Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods attempts to address the increasingly important need of straddling species boundaries in the context of a single research program by compiling research protocols used in a wide range of vertebrate species. In fact, this volume has been designed so that readers can readily find information on species other than the one with which they may be most familiar. These protocols include not only embryological methods, but also cellular and genetic approaches that have complemented and expanded our understanding of embryonic development. In addition, a number of chapters highlight a specific method that is in principle applicable to multiple species, such as TILLING and ZFN-mediated mutagenesis, the generation of Embryonic Stem (ES) cell lines, and nuclear/oocyte transfer. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods serves as an ideal guide to the molecular, cell, and developmental biology community and will hopefully contribute to the ongoing collective effort towards a better understanding of the beauty and logic of vertebrate development.

For many years, Springer has been publishing an impressive series of textbooks of pharmacology which have set standards in medical science. Surprisingly, an extensive overview of the current state of the art in research on estrogens and anti estrogens was still lacking. The present two volumes on estrogens and antiestrogens provide a comprehensive review of a field of research in which remarkable progress has been made over the past few years. New insights into the mechanisms of steroid hormone action resulted in a tremendous number of publications from which new principles of preventive and therapeutic appli cations of estrogens and antiestrogens emerged. Although various electronic data bases provide easy access to this copious information, there was a clear necessity for a monograph-style textbook which assesses and summarizes current knowledge in this rapidly expanding field of research. It should be noted, however, that, due to this dynamic development, it is barely possible to comprehensively update every aspect of basic and clinical knowledge on estro gens and antiestrogens. Thus, the intention of the editors was to provide the reader with an overview of the "classic" and most recently explored areas of research and stimulate future interests in basic and applied endocrinology. Estrogens were among the first steroid hormones described in the scientific literature. Since they were first isolated, since the chemical, synthesic and pharmacological characterization of naturally occurring estrogens and, later on, of orally active derivatives, estrogen research has produced continu ously hallmark results in reproductive endocrinology worldwide.

Proceedings of the European Cooperation in the Field of Scientific and Technical Research (COST 825) Symposium on Mammary Gland Biology, held September 16-18, 1999, in Tours, France. It is difficult to overstate the evolutionary and functional significance of mammary tissue in biology. Substantial progress has been made by researchers in various disciplines, particularly over the last fifteen years, towards realizing the potential of this tissue to yield powerful experimental models for morphogenesis and tissue development; for cellular differentiation; for the biosynthesis and secretion of proteins, lipids, small molecules and inorganic salts; and for the coordination and regulation of these processes. More recently, the possibility of exploiting the secretory epithelial cells of mammary tissue as cell factories' has become a reality and the recombinant production by lactating animals of an increasing number of proteins, valuable both in the pharmaceutical and nutraceutical' fields, is in progress or under development. Also in this sphere of agricultural production, genetic as well as nutritional technologies are under investigation and exploitation to optimize milk composition for various end-uses - for instance in food process and manufacture. The possibilities of deriving health benefit from the bioactive properties of some of the minor constituents of milk are emerging to counter the highly-publicized negative health impact of excessive consumption of saturated animal fats. In human nutrition and medicine, the mammary gland is both a source of nutrition to the neonate and a potential health threat to the adult female - breast cancer remains the major single cause of female mortality in most developed countries. This volume provides a unique glimpse into our understanding, at the cutting edge of a variety of disciplines, of this versatile and extraordinary tissue, at the birth of the twenty-first century.

This book examines the biology of tuco-tucos (Ctenomys) from an evolutionary perspective. Historically, these subterranean rodents have long attracted the attention of scientists due to its remarkable chromosomes variability and rapid diversification. A wealth of knowledge on physiology, ecology, genetics, morphology, paleontology, and taxonomy has been documented in the last 70 years through numerous single publications. In this volume, expert investigators review and frame these essays with the breadth of current understanding. The collection of chapters are presented into the major topics: i) Evolution of Ctenomys, ii) Geographic Patterns, iii) Organismal Biology, and iv) Environmental Relationships. Given its scope, the book will be of interest to both students and researchers and may stimulate further research with this exciting model on a wide range of evolutionary topics.

This book aims to be a comprehensive review of the literature on the conservation genetics of the New World crocodilians, from the biological and demographical aspects of the living species to the application of molecular techniques for conservation purposes. It covers the current status of the molecular genetics applied to phylogenetics, phylogeography, diversity, kinship and mating system, and hybridization, as well its implications for decision making with regards to the conservation of these species at academic and governmental levels. This book can be used as a guide for graduate and undergraduate students to understand how conservation genetics techniques are carried out and how they can help preserve not only crocodilians but also other living species.

Population genomics is revolutionizing wildlife biology, conservation, and management by providing key and novel insights into genetic, population and landscape-level processes in wildlife, with unprecedented power and accuracy. This pioneering book presents the advances and potential of population genomics in wildlife, outlining key population genomics concepts and questions in wildlife biology, population genomics approaches that are specifically applicable to wildlife, and application of population genomics in wildlife population and evolutionary biology, ecology, adaptation and conservation and management. It is important for students, researchers, and wildlife professionals to understand the growing set of population genomics tools that can address issues from delineation of wildlife populations to assessing their capacity to adapt to environmental change. This book brings together leading experts in wildlife population genomics to discuss the key areas of the field, as well as challenges, opportunities and future prospects of wildlife population genomics.