This book is about the philosophy of de-extinction. To make an extinct species 'de-extinct' is to resurrect it by creating new organisms of the same, or similar, appearance and genetics. The book describes current attempts to resurrect three species, the aurochs, woolly mammoth and passenger pigeon. It then investigates two major philosophical questions such projects throw up. These are the Authenticity Question-'will the products of de-extinction be authentic members of the original species?'-and the Ethical Question-'is de-extinction something that should be done?' The book surveys and critically evaluates a raft of arguments for and against the authenticity or de-extinct organisms, and for and against the ethical legitimacy of de-extinction. It concludes, first, that authentic de-extinctions are actually possible, and second, that de-extinction can potentially be ethically legitimate, especially when deployed as part of a 'freeze now and resurrect later' conservation strategy.

This book describes the discovery of molecules from unexploited extreme marine environments, and presents new approaches in marine genomics. It combines the current state of knowledge in marine genomics and advanced natural products' chemistry to pursue the sustainable production of novel secondary metabolites (lead compounds), as well as pharmacologically active peptides/proteins, with antimicrobial, neuroprotective, anti-osteoporotic, anti-protozoan/anti-plasmodial, anti-ageing and immune-modulating effects. Further, it employs molecular-biology-based approaches and advanced chemical techniques to obtain and to select candidate compounds for pre-clinical and clinical studies.

This book presents a concept for implementing a mass balance approach toward developing an effective eco-friendly, livestock farming system independent of external energy input. In this context it describes a modern, integrated farming system, and includes comprehensive technical information explaining the design and evaluation of manure management systems, and modeling and operational tools. It first discusses the mass balance operating process, highlighting the difference between imported and exported mass across the farm boundary. Estimating mass balance can provide critical information for (comprehensive) nutrient management planning and for managing the movement of nutrients and manure. It then explains the estimation of whole-farm P mass balance using a suitable model system. The subsequent chapters provide updated information on management aspects of livestock-farming and generation of multiple job opportunities, and also explore various aspects of livestock farming operational protocols like housing and management; nurture of rams, ewes and lambs, new born calves and heifers; care of buck, doe and kid- nutrition flushing; concept zero grazing-systems; disease control and management; integrated goat farming; and crop-livestock integration. Further, the book addresses crop-livestock integration; energy autonomy in cattle farming; value added biopharmaceuticals from cattle farming; CAPEX for cattle farming; concepts of cattle farming; detrimental effects of the industry; topographic and edaphic factors, and thermal stress on livestock growth and development; socioeconomic development; and water requirements for livestock. The book concludes with the most important issue in the field of agriculture and veterinary science: "Livestock Farming with Care," describing sustainable, eco-friendly livestock farming by highlighting issues like animal feed vs. human food; agricultural GDP vs livestock, and factors affecting the sustainability of livestock farming. Given its scope, this book is a valuable resource for researchers and students alike, and will also appeal to practitioners in the field of livestock.

This book, written by experienced geneticists, covers topics ranging from the natural history of the mouse species, its handling and reproduction in the laboratory, and its classical genetics and cytogenetics, to modern issues including the analysis of the transcriptome, the parental imprinting and X-chromosome inactivation. The strategies for creating all sorts of mutations, either by genetic engineering or by using mutagens, are also reviewed and discussed in detail. Finally, a last chapter outlines the methodology used for the analysis of complex or quantitative traits. The authors also discuss the importance of accurate phenotyping, which is now performed in the mouse clinics established worldwide and identify the limits of the mouse model, which under certain circumstances can fail to present the phenotype expected from the cognate condition in the human model. For each chapter an up-to-date list of pertinent references is provided. In short, this book offers an essential resource for all scientists who use or plan to use mice in their research.

Drosophila melanogaster (fruit fly) is a highly versatile model with a genetic legacy of more than a century. It provides powerful genetic, cellular, biochemical and molecular biology tools to address many questions extending from basic biology to human diseases. One of the most important questions in biology is how a multi-cellular organism develops from a single-celled embryo. The discovery of the genes responsible for pattern formation has helped refine this question and has led to other questions, such as the role of various genetic and cell biological pathways in regulating the process of pattern formation and growth during organogenesis. The Drosophila eye model has been extensively used to study molecular genetic mechanisms involved in patterning and growth. Since the genetic machinery involved in the Drosophila eye is similar to humans, it has been used to model human diseases and homology to eyes in other taxa. This updated second edition covers current progress in the study of molecular genetic mechanisms of pattern formation, mutations in axial patterning, genetic regulation of growth, and more using the Drosophila eye as a model.

This book explains omics at the most basic level, including how this new concept can be properly utilized in molecular and systems biology research. Most reviews and books on this topic have mainly focused on the technicalities and complexity of each omics' platform, impeding readers to wholly understand its fundamentals and applications. This book tackles such gap and will be most beneficial to novice in this area, university students and even researchers. Basic workflow and practical guidance in each omics are also described, such that scientists can properly design their experimentation effectively. Furthermore, how each omics platform has been conducted in our institute (INBIOSIS) is also detailed, a comprehensive example on this topic to further enhance readers' understanding. The contributors of each chapter have utilized the platforms in various manner within their own research and beyond. The contributors have also been interactively integrated and combined these different omics approaches in their research, being able to systematically write each chapter with the conscious knowledge of other inter-relating topics of omics. The potential readers and audience of this book can come from undergraduate and postgraduate students who wish to extend their comprehension in the topics of molecular biology and big data analysis using omics platforms. Furthermore, researchers and scientists whom may have expertise in basic molecular biology can extend their experimentation using the omics technologies and workflow outlined in this book, benefiting their research in the long run.

The study of alternative reproductive tactics (the behavioural strategies used by individuals to increase their reproductive success) is an evolutionary puzzle, and one of great interest to researchers. For instance, why do some males guard both nest and eggs, while others sneak into nests while pairs are spawning and fertilise those eggs? The field offers a special opportunity to study the evolution and functional causes of phenotypic variation, which is a general problem in the field of evolutionary biology. By integrating both mechanistic (psychological) and evolutionary (behavioural ecology) perspectives and by covering a great diversity of species, Alternative Reproductive Tactics addresses this integrated topic of longstanding interest, bringing together a multitude of otherwise scattered information in an accessible form that is ideal for graduate students and researchers.

This is a reference handbook for young researchers exploring gene and cell therapy. Gene therapy could be defined as a set of strategies modifying gene expression or correcting mutant/defective genes through the administration of DNA (or RNA) to cells, in order to treat disease. Important advances like the discovery of RNA interference, the completion of the Human Genome project or the development of induced pluripotent stem cells (iPSc) and the basics of gene therapy are covered. This is a great book for students, teachers, biomedical researchers delving into gene/cell therapy or researchers borrowing skills from this scientific field.

The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology (TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state of the art, Transgenic Mouse Methods and Protocols, second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.

Echinoderms and prochordates occupy a key position in vertebrate evolution. The genomes of sea urchin share 70% homology with humans. Researches on cell cycle in sea urchin and phagocytosis in asteroids have fetched Nobel Prizes. In this context, this book assumes immense importance. Echinoderms are unique, as their symmetry is bilateral in larvae but pentamerous radial in adults. The latter has eliminated the development of an anterior head and bilateral appendages. Further, the obligate need to face the substratum for locomotion and acquisition of food has eliminated their planktonic and nektonic existence. Egg size, a decisive factor in recruitment, increases with decreasing depths up to 2,000-5,000 m in lecithotrophic asteroids and ophiuroids but remains constant in their planktotrophics. Smaller (< 18 mm) ophiuroids do not produce a lecithotrophic eggs but larger (> 110 mm) asteroids generate planktotrophic eggs only. Publications on sex ratio of echinoderms indicate the genetic determination of sex at fertilization but those on hybridization, karyotype and ploidy induction do not provide evidence for heterogametism. But the herbivorous echinoids and larvacea with their gonads harboring both germ cells and Nutritive Phagocytes (NPs) have economized the transportation and hormonal costs on gonadal function. Despite the amazing potential just 2 and 3% of echinoderms undergo clonal reproduction and regeneration, respectively. Fission is triggered, when adequate reserve nutrients are accumulated. It is the most prevalent mode of clonal reproduction in holothuroids, asteroids and ophiuroids. However, budding is a more prevalent mode of clonal reproduction in colonial hemichordates and urochordates. In echinoderms, fission and budding eliminate each other. Similarly, autoregulation of early development eliminates clonal reproduction in echinoids and solitary urochordates. In pterobranchs, thaliaceans and ascidians, the repeated and rapid budding leads to colonial formation. Coloniality imposes reductions in species number and body size, generation time and life span, gonad number and fecundity as well as switching from gonochorism to simultaneous hermaphorditism and oviparity to ovoviviparity/viviparity.

The first volume in the new Cambridge Handbooks in Behavioral Genetics series, Behavioral Genetics of the Mouse provides baseline information on normal behaviors, essential in both the design of experiments using genetically modified or pharmacologically treated animals and in the interpretation and analyses of the results obtained. The book offers a comprehensive overview of the genetics of naturally occurring variation in mouse behavior, from perception and spontaneous behaviors such as exploration, aggression, social interactions and motor behaviors, to reinforced behaviors such as the different types of learning. Also included are numerous examples of potential experimental problems, which will aid and guide researchers trying to troubleshoot their own studies. A lasting reference, the thorough and comprehensive reviews offer an easy entrance into the extensive literature in this field, and will prove invaluable to students and specialists alike.

The use of ultrasonic imaging techniques for diagnostic purposes in veteri nary medicine and animal science has lagged far behind their use in human medicine. In the area of domestic animal reproduction, diagnostic ultrasono graphy has a relatively short history. Reports on B-mode scanning first appeared as late as 1969, when Stouffer and co-workers used it for counting foetal numbers in late gestation sheep. After Lindahl had re-evaluated the potential of two-dimensional ultrasonography for pregnancy diagnosis in sheep in 1976, results of the first large scale field-trials by Fowler and Wilkins on predicting foetal numbers during the first half of gestation in sheep were published as recently as 1980. In this year also the first paper, by Palmer and co-workers, on ultrasonic scanning of the uterus and ovaries in the mare appeared. The improvement of diagnostics which was achieved by the application of this technique is illustrated by its present routine use in clinical equine practice and sheep breeding. Besides offe ring an accurate early pregnancy diagnosis in species such as the horse, cow, sheep, goat, pig and dog, ultrasonography enables the visualization of ovarian and uterine structures for the identification of both physiological and pathological conditions. In this way it replaces or supplements the more common diagnostic techniques used by the general practitioner so far. Ultrasonography has also opened several new lines of research for clini cians and research workers."

The aim of this book is to systematize and discuss population genetic studies of freshwater fish in a region that harbors the greatest diversity of species among all inland water ecosystems. This volume explores the genetic evaluation for a number of orders, families and species of Neotropical fishes, and provides an overview on genetic resources and diversity and their relationships with fish domestication, breeding, and food production.

Defined as, "The science about the development of an embryo from the fertilization of the ovum to the fetus stage," embryology has been a mainstay at universities throughout the world for many years. Throughout the last century, embryology became overshadowed by experimental-based genetics and cell biology, transforming the field into developmental biology, which replaced embryology in Biology departments in many universities. Major contributions in this young century in the fields of molecular biology, biochemistry and genomics were integrated with both embryology and developmental biology to provide an understanding of the molecular portrait of a "development cell." That new integrated approach is known as stem-cell biology; it is an understanding of the embryology and development together at the molecular level using engineering, imaging and cell culture principles, and it is at the heart of this seminal book. Stem Cells and Regenerative Medicine: From Molecular Embryology to Tissue Engineering is completely devoted to the basic developmental, cellular and molecular biological aspects of stem cells as well as their clinical applications in tissue engineering and regenerative medicine. It focuses on the basic biology of embryonic and cancer cells plus their key involvement in self-renewal, muscle repair, epigenetic processes, and therapeutic applications. In addition, it covers other key relevant topics such as nuclear reprogramming induced pluripotency and stem cell culture techniques using novel biomaterials. A thorough introduction to stem-cell biology, this reference is aimed at graduate students, post-docs, and professors as well as executives and scientists in biotech and pharmaceutical companies.

This open access book summarizes the latest scientific findings regarding the biological effects of the Fukushima Daiichi Nuclear Power Plant (FNPP) accident in 2011. Various cases of changes in animals and organisms have been reported since the FNPP accident. However, it is often unknown whether they are actually due to radiation, since the dose or dose-rate are not necessarily associated with the changes observed. This book brings together the works of radiation biologists and ecologists to provide reliable radioecology data and gives insight into future radioprotection. The book examines the environmental pollution and radiation exposure, and contains valuable data from abandoned livestock in the ex-evacuation zone and from wild animals including invertebrates and vertebrates, aqueous and terrestrial animals, and plants that are subjected to long-term exposure in the area still affected by radiation. It also analyzes dose evaluation, and offers new perspectives gained from the accident, as well as an overview for future studies to promote radioprotection of humans and the ecosystem. Since the biological impact of radiation is influenced by various factors, it is difficult to scientifically define the effects of low-dose/low-dose-rate radiation. However, the detailed research data presented can be combined with the latest scientific and technological advances, such as artificial intelligence, to provide new insights in the future. This book is a unique and valuable resource for researchers, professionals and anyone interested in the impact of exposure to radiation or contamination with radioactive materials.

This collection of essays looks at sexuality and reproduction from an evolutionary perspective. Covering experimental discoveries as well as theoretical investigations, the volume explores the relationship between evolution and other areas of human behaviour.

This book is open access under a CC BY 4.0 license This open access book brings together the latest genome base prediction models currently being used by statisticians, breeders and data scientists. It provides an accessible way to understand the theory behind each statistical learning tool, the required pre-processing, the basics of model building, how to train statistical learning methods, the basic R scripts needed to implement each statistical learning tool, and the output of each tool. To do so, for each tool the book provides background theory, some elements of the R statistical software for its implementation, the conceptual underpinnings, and at least two illustrative examples with data from real-world genomic selection experiments. Lastly, worked-out examples help readers check their own comprehension.The book will greatly appeal to readers in plant (and animal) breeding, geneticists and statisticians, as it provides in a very accessible way the necessary theory, the appropriate R code, and illustrative examples for a complete understanding of each statistical learning tool. In addition, it weighs the advantages and disadvantages of each tool.

Reproductive Biology and Phylogeny of Lizards and Tuatara is a remarkable compendium of chapters written by the world's leading experts from over four continents. The book begins with a chapter recounting historical discoveries in reproductive biology and a review of phylogenetics and up-to-date hypotheses concerning evolutionary relationships among lizards. Following these chapters are detailed reviews with additional new data concerning chemical communication, sexual selection, reproductive cues, female reproductive anatomy, female reproductive cycles, oogenesis, parthenogenesis, male reproductive anatomy, male reproductive cycles, spermatogenesis, reproductive investment, viviparity and placentation, multiple paternity, and parental care. The book culminates in two chapters on tuatara reproduction giving unique insight into evolutionary patterns in reproductive biology in squamates and tuatara. This is an essential resource for anyone studying reproduction in reptiles and/or vertebrates and offers a fascinating read for those interested in reproductive biology.

This collection of essays looks at sexuality and reproduction from an evolutionary perspective. Covering experimental discoveries as well as theoretical investigations, the volume explores the relationship between evolution and other areas of human behaviour.

About 95 per cent of all known animal species are invertebrates. A knowledge of their sexual, reproductive, and developmental biology is essential for the effective management of species that are economically useful to man or are harmful to him, his crops, and livestock. This treatise is the first to cover all aspects of reproduction and development of the entire spectrum of invertebrates -- terrestrial, marine, freshwater, brackish-water, free-living, and parasitic. The chapters, by leading world experts in their fields, are up-to-date and informative, and suggest a number of problems for future research. Asexual Propagation and Reproductive Strategies, issued in two parts, is the sixth volume in the series. Part A: Porifera through Mollusca Contents Series Preface; Preface to Volume VI; Systematic Resume of the Invertebrates; Porifera, Paul E. Fell; Cnidaria, Stanley Shostak; Platyhelminthes-Turbellaria, Mario Benazzi and Giuseppina Benazzi Lentati; Platyhelminthes-Trematoda, Roy M. Anderson; Platyhelminthes-Eucestoda, Larry S. Roberts and C. R. Kennedy; Mesozoa, Bayard H. McConnaughey; Rotifera, John J. Gilbert; Gastrotricha, M. R. Hummon and W. D. Hummon; Acanthocephala, C. R. Kennedy; Sipuncula, Mary E. Rice and John F. Pilger, Mollusca, N. W. Runham; Species Index; Subject Index. Part B: Annelida-Clitellata through Cephalochordata Contents Series Preface; Preface to Volume VI; Systematic Resume of the Invertebrates; Annelida-Clitellata, B. Christensen; Tardigrada, Roberto Bertolani; Arthropoda-Crustacea, T. J. Pandian; Arthropoda-lnsecta, J. Muthukrishnan; Pentastomida, J. Riley; Bryozoa Entoprocta, C. Nielsen; Brachiopoda, S. H. Chuang; Chaetognatha, A. Alvarino; Echinodermata: Asexual Propagation, Philip V. Mladenov and R. D. Burke; Hemichordata, J. A. Petersen; Cephalochordata, Jaypaul Azariah; Species Index; Subject Index.

Offering coverage of a wide range of topics on snake reproduction and phylogeny, this comprehensive book discusses everything from primordial germ migration in developing embryos to semelparity (death after reproduction) in the aspic viper. Beginning with a review of the history of snake reproductive studies, it presents new findings on development, placentation, spermatogenesis, male and female reproductive anatomy, hormonal control of reproduction, reproductive cycles, sex pheromones, and parental care. An indispensible reference, this book offers comparative chapters on snake phylognetics examining morphological characteristics alongside strictly molecular concerns. It is rife with illustrations and color plates.

Dr. Anjali Aggarwal is working as a Senior Scientist at National Dairy Research Institute, Karnal (India). She holds a PhD degree in Animal Physiology and is involved in research and teaching at post-graduate level. Her area of research work is stress and environmental physiology. She has more than 50 publications, two technical bulletins, four manuals and many book chapters to her credit. She has successfully guided many post-graduate and PhD students. Her major research accomplishments are on microclimatic modification for alleviation of heat and cold stress, mist and fan cooling systems for cows and buffaloes, and use of wallowing tank in buffaloes. Her work involves the use of technology of supplementing micronutrients during dry period and early lactation to crossbred and indigenous cows for alleviating metabolic and oxidative stress and improved health and productivity. Studies are also done in her lab on partitioning of heat loss from skin and pulmonary system of cattle and buffaloes as a result of exercise or exposure to heat stress. Dr. R.C. Upadhyay is working as Head, Dairy Cattle Physiology Division at National Dairy Research Institute, Karnal (India). He graduated in Veterinary Sciences and obtained his PhD degree in Animal Physiology. His area of recent research is climate change, stress, and environmental physiology. His major research accomplishment is on climate change impact assessment of milk production and growth in livestock. His work also involves studying methane conversion and emission factors for Indian livestock and use of IPCC methodology of methane inventory of Indian livestock. Heat shock protein-70 expression studies in cattle and buffaloes are also done in his lab. Draught animal power evaluation, fatigue assessment, work-rest cycle and work limiting factors form the highlights of his work. Studies on partitioning of heat loss from skin and pulmonary system of cattle and buffaloes and electrocardiographic studies in cattle, buffalo, sheep and goat are also undertaken in his lab. He has more than 75 research papers, four books and several book chapters to his credit. Technologies developed and research done by him include methodology of methane measurement: open and closed circuit for cattle and buffaloes; inventory of methane emission from livestock using IPCC methodology; livestock stress index: thermal stress measurement based on physiological functions; and draught power evaluation system and large animal treadmill system. He received training in Radio-nuclides in medicine at Australian School of Nuclear Technology, Lucas heights, NSW, Australia in 1985 and Use of radioisotopes in cardiovascular investigations at CSIRO, Prospect, NSW, Australia, during 1985-86. He has guided several post-graduate and PhD students. He is recipient of Hari Om Ashram Award-1990 (ICAR) for outstanding research in animal sciences.

This book represents the first analysis of the evolutionary significance of sperm phenotypes and derived sperm traits and the possible selection pressures responsible for sperm-egg coevolution.
An understanding of sperm evolution is fast developing and promises to shed light on many topics from basic reproductive biology to the evolutionary process itself as well as the sperm proteome, the sperm genome and the quantitative genetics of sperm. The Editors have identified 15 topics of current interest and biological significance to cover all aspects of this bizarre, fascinating and important subject. It comprises the most comprehensive and up to date review of the evolution of sperm, and pointers for future research, written by experts in both sperm biology and evolutionary biology. The combination of evolution and sperm is a potent mix and this is the definitive account.
* The first review survey of this emerging field
* Written by experts from a broad array of disciplines from the physiological and biomedical to the ecological and evolutionary
* Sheds light on the intricacies of reproduction and the coevolution of sperm, egg and reproductive behaviour

This concise but thorough study of courtship behavior in fish, birds, and arthropods is the first rigorous examination of the evolutionary origins and mechanisms of courtship and its contribution to biological success. Demonstrating the fruitfulness of an empirically based, inductive approach to understanding courtship, the book also explains clearly how principles of modern evolutionary theory can be successfully employed in studying behavior.

The author describes many observations and experiments that have not previously appeared outside specialized journals and brings an abundance of simple yet accurate examples of animal behavior to bear on explanations of ethological concepts and evolutionary theory. No attempt is made to skim over the gaps of knowledge apparent in the study of behavior evolution; rather, the author discusses the limitations and difficulties of different approaches, critically reviews the deductions that can be and have been made from them, and tries to present enough evidence on controversial points for the reader himself to judge the validity of specific arguments.

Indicating how ethological method, firmly based on biological principles, can intensively investigate and illuminate a single area of animal behavior, the book will be valuable to students and professionals in zoology, animal behavior, and experimental psychology.

"Margaret Bastock" studied zoology at Oxford from 1946 to 1949 and became a zoology tutor at and, later, a Fellow of St. Anne's College, Oxford. She worked for her Ph.D. under Professor N. Tinbergen and through him became acquainted with the work of the chief ethologists in Europe and the United States. Her original work has concentrated on the inheritance and organization of behavior in insects, and her principal interests include all aspects of animal behavior, genetics, and evolution.

One of the little known species in the danger of extinction, gymophionan amphibians, are also known as caecilians or apoda. Gymnophiona contains 154 species belonging to 34 genera and 6 families. For many years, studies on the Gymnophiona were disparate and still only a few species have been deeply studied. Fortunately, in recent years, some new works have been published on their systematics, using both the classical methods as well as immunology and molecular biology. New data have also been obtained on the biology, life history, reproductive biology, endocrinology and embryonic development of several species. These fascinating aspects along with other important ones on gymnophionan studies are ably reviewed in this book.