This second edition provides 21 new chapters on methods used in
laboratories for investigating the physiology and molecular
genetics of the pathogen Clostridium difficile. Chapters detail
up-to -date experimental techniques for gene editing and
transcriptional analysis which are used to investigate the
fundamental biology of the organism and its virulence factors.
Additional chapters describe development of potential new
treatments including vaccines, bacteriophage and faecal
transplantation. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Clostridium difficile: Methods and
Protocols, Second Edition provides a comprehensive catalogue of
molecular tools and techniques authored by the researchers who have
developed them.
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