Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical)
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Atomic Force Microscopy - A New Look at Microbes (Hardcover)
Loot Price: R1,862
Discovery Miles 18 620
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Atomic Force Microscopy - A New Look at Microbes (Hardcover)
Series: Synthesis Lectures on Materials and Optics
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Over the last two decades, Atomic Force Microscopy (AFM) has
undoubtedly had a considerable impact in unraveling the structures
and dynamics of microbial surfaces with nanometer resolution, and
under physiological conditions. Moreover, the continuous
innovations in AFM-based modalities as well as the combination of
AFM with modern optical techniques, such as confocal fluorescence
microscopy or Raman spectroscopy, increased the diversity and
volume of data that can be acquired in an experiment. It is evident
that these combinations provide new ways to investigate a broad
spectrum of microbiological processes at the level of single cells.
In this book, I have endeavored to highlight the wealth of
AFM-based modalities that have been implemented over the recent
years leading to the multiparametric and multifunctional
characterization of, specifically, bacterial surfaces. Examples
include the real-time imaging of the nanoscale organization of cell
walls, the quantification of subcellular chemical heterogeneities,
the mapping and functional analysis of individual cell wall
constituents, and the probing of the nanomechanical properties of
living bacteria. It is expected that in the near future more
AFM-based modalities and complementary techniques will be combined
into single experiments to address pertinent problems and
challenges in microbial research. Such improvements may make it
possible to address the dynamic nature of many more microbial cell
surfaces and their constituents, including the restructuring of
cellular membranes, pores and transporters, signaling of cell
membrane receptors, and formation of cell-adhesion complexes.
Ultimately, manifold discoveries and engineering possibilities will
materialize as multiparametric tools allow systems of increasing
complexity to be probed and manipulated.
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