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Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Neurosciences
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Patch-Clamp Applications and Protocols (Paperback, Softcover reprint of the original 1st ed. 1995)
Loot Price: R2,966
Discovery Miles 29 660
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Patch-Clamp Applications and Protocols (Paperback, Softcover reprint of the original 1st ed. 1995)
Series: Neuromethods, 26
Expected to ship within 10 - 15 working days
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E. Neher and B. Sakman were the first to monitor the opening and
closing of single ion channels and membranes by conductance
measurements. In 1976, they used firepolished micropipets with a
tip diameter of 3-5 pm to record currents from a small patch of the
membranbe of sk- etal muscles, thereby decreasing background
membrane noise. In order to reduce the dominant source of
background noise-the leakage shunt under the pipet rim between m-
the muscle membrane had to be treated brane and gla- enzymatically.
Despite these early limitations, a new te- nique was born -the
patch-clamp technique. The final bre- through came in 1981 when the
same authors, in collaboration with 0. P. Hamill, A. Marty, and F.
J. Sigworth, developed the gigaohm seal. Not only did this improve
the quality of recordings, it was now possible to gently pull the
membrane patch with the attached pipet off the cell and study its
trapped ion channels in isolation. Another offshoot of the gigaohm
seal technique was the whole-cell patch-clamp technique, in which
the patch is ruptured without breaking the seal. This technique is
really a sophisticated voltage-clamp technique and also allows for
the altering of cytoplasmic constituents if the experimenter so
wishes. The first part of Patch-Clamp Applications and Protocols
presents modern developments associated with the techn- ogy of
patch-clamp electrodes, of cell-free ion channel reco- ing, and of
the whole-cell patch-clamp technique.
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