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Ultraviolet Spectroscopy of Proteins (Paperback, Softcover reprint of the original 1st ed. 1986)
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Ultraviolet Spectroscopy of Proteins (Paperback, Softcover reprint of the original 1st ed. 1986)
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The aim of this book is to give a comprehensive description of the
basic methods used in the ultraviolet spectroscopy of proteins, to
discuss new trends and development of these methods, and to analyze
their different applications in the study of various aspects of
protein structure and dynamics. Ultraviolet spectroscopy is one of
the oldest and most popular methods in the field of biochemistry
and molecular biophysics. At present, it is difficult to imagine
the biochemical laboratory without a recording spectrophotometer or
spectrofluorimeter. There are several hundreds of publications
directly devoted to protein ultraviolet spectroscopy and in a great
number of studies UV spectroscopic methods are used for the
structural analysis of different proteins. Meanwhile a unified
description of the theoretical basis of the methods, experimental
techniques, data analysis, and generalization of results obtained
in solving the specific problems of protein structure are lacking.
There are three reasons for which a monograph on ultraviolet
spectroscopy is needed today. Firstly, there has been significant
growth in facilities of experimental technique, its precision, and
versatility associated with computer data analysts. This new
technique is available to a wide circle of scientists engaged in
the field of protein research. Most of them are not spectroscopists
and, thus, there is a need for a conceivable and precise source of
information on how to use this method and what kind of data it
should provide.
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