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Advances in Neurochemistry - Volume 3 (Paperback, Softcover reprint of the original 1st ed. 1978)
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Advances in Neurochemistry - Volume 3 (Paperback, Softcover reprint of the original 1st ed. 1978)
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The original premise of the Editors in initiating this series was
that there existed a readership ofneurochemists with considerable
biochemical back ground who would make use of a series dedicated to
both new develop ments and specialized reviews in neurochemistry.
Having selected our authors, we have offered them virtually
complete freedom to reflect and speculate in a field in which they
have achieved prominence. The response to the first two volumes has
been rewarding. The present one continues in this tradition. While
we have not attempted to publish specialized volumes, the present
volume contains two somewhat related chapters (Chapters 4 and 5, on
the role of amino acid neurotransmitters). The first three chapters
examine three diverse approaches, each of current interest, in
neurochemi cal approaches to the molecular bases of neuronal and
glial structure. B. W. Agranoff M. H. Aprison vii CONTENTS CHAPTER
1 2',3'-CYCUC NUCLEOTIDE 3'-PHOSPHODIESTERASE NEIL RAYMOND SIMS AND
PATRICK ROBERT CARNEGIE 1. Introduction. . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2.
Assay of CNPase . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . 3 2. 1. Need for Activation. . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . 3 2. 2.
Comparison of Assays . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . 3 3. Association of CNPase with Myelin . . . . . . .
. . . . . . . . . . . . . . . . 7 3. 1. Historical. . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3. 2. Subcellular Fractionation. . . . . . . . . . . . . . . . . .
. . . . . . . . . . 8 3. 3. Development. . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . 8 3. 4. Mutant Mice
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . 9 3. 5. Use as a Myelin Marker . . . . . . . . . . . . . .
. . . . . . . . . . . . . . 10 3. 6. Myelin-Related Fractions and
Peripheral Nerve Myelin. 11 4. CNPase in Nonmyelin Fractions. . . .
. . . . . . . . . . . . . . . . . . . . . . 13 5. Activation and
Isolation. . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . 13 5. 1. Introduction. . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . 13 5. 2. Activation. . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . 14 5. 3. Solubilization and Fractionation. . . . . . . . .
. . . . . . . . . . . . . 15 6. Properties. . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
19 6. 1. Substrate Specificity. . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . .
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