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Rhodopsin - Methods and Protocols (Hardcover, 2015 ed.)
Loot Price: R3,102
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Rhodopsin - Methods and Protocols (Hardcover, 2015 ed.)
Series: Methods in Molecular Biology, 1271
Expected to ship within 10 - 15 working days
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This volume explores the technological improvements in protein
engineering, expression, purification, and crystallization of
several rhodopsin photoactive intermediates, thus increasing our
understanding of rhodopsin activation. The first chapters of the
book focus on methods developed to study fundamentals of rhodopsin
structure and function, starting with improved purification
protocols of native and mutated rhodopsin, followed by methods used
for rhodopsin reconstitution into lipid bilayers stabilizing
rhodopsin function properties, and finally describing recently
developed methods to study structural dynamics of rhodopsin
activation and its mechanistic properties. Subsequently, chapters
underline various techniques that have been developed to visualize
the rhodopsin dimer and to study its functional significance. The
next few chapters highlight cutting-edge imaging techniques of
photoreceptors, rhodopsin trafficking, and its diffusion within
signaling membranes. Finally the book concludes with recent
developments that could be potentially beneficial in patient
treatments, and treatment strategies for retinal degenerative
diseases. Written in the highly successful Methods in Molecular
Biology series format, the chapters include the kind of detailed
description and implementation advice that is crucial for getting
optimal results in the laboratory. Timely and practical, Rhodopsin:
Methods and Protocols reaches out to researchers and health
practitioners, and provides timely protocol useful for studying
structural and functional properties of rhodopsin.
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