Only in recent times has the possibility of growing and
implanting replacement teeth, made from one s own cells, moved into
the realm of realistic possibilities; however, the molecular and
cellular mechanisms of tooth development must be studied in a range
of vertebrates, from zebrafish to mice, so that evolutionarily
conserved network kernels, which will define the cellular states of
generic vertebrate tooth development, can be recognized. In
"Odontogenesis: Methods and Protocols," experts in the field
examine techniques to approach this burgeoning field. This detailed
volume includes chapters on the detection of tooth development gene
expression, both at the RNA and protein level, current approaches
to the manipulation of gene expression levels and subsequent
analysis of tooth phenotypes, as well as chapters concerning
current efforts to get living tooth implants working without
waiting for a full understanding of the developmental pathways at
the molecular level. Written in the highly successful "Methods in
Molecular Biology " series format, chapters include introductions
to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips for troubleshooting and avoiding known pitfalls.
Practical and easy to use, "Odontogenesis: Methods and
Protocols" aims to help researchers move forward toward the
ultimate goal of getting a bioengineered tooth into the patient s
mouth.
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