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Books > Medicine > Clinical & internal medicine > Diseases & disorders > Infectious & contagious diseases
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Diagnosis of Sexually Transmitted Diseases - Methods and Protocols (Hardcover, 2012 ed.)
Loot Price: R3,047
Discovery Miles 30 470
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Diagnosis of Sexually Transmitted Diseases - Methods and Protocols (Hardcover, 2012 ed.)
Series: Methods in Molecular Biology, 903
Expected to ship within 10 - 15 working days
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Sexually transmitted infections (STI) continue to be a major cause
of morbidity and mortality, both in developed industrial countries
as well as in the developing world. Human immunodeficiency virus
infections and the ensuing opportunistic infections are a major
drain on the human and financial resources of many countries in the
developing world and even with the availability of effective
treatment the epidemic is not yet contained. Screening in
developing worlds is difficult and there is a need for simple
reliable cheap diagnostic methods that can be performed at the
point of care, on the same day and by staff with limited training.
Molecular biological methods are very attractive for the diagnosis
of STI since a well defined range of pathogens is responsible for
the infection. Diagnosis of Sexually Transmitted Diseases: Methods
and Protocols strives to cover the full range of molecular testing
for STI. Chapters cover a variety of topics such as aspects of DNA
extraction from small volume samples or difficult tissues, simple,
nested or multiplex PCR, use of duplex primers or other
modifications of primers and PCR conditions, sequence analysis for
genotyping, denaturing gel analysis, microarrays using liquid beads
or microspheres and silicon nanoparticle-enhanced microcantilever
detection of DNA. Written in the successful Methods in Molecular
Biology (TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative and
easily accessible, Diagnosis of Sexually Transmitted Diseases:
Methods and Protocols will be a valuable reference for the
scientist looking to establish molecular methodologies for the
diagnosis of STI tests in their own lab.
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