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Books > Professional & Technical > Biochemical engineering > Biotechnology
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Microinjection - Methods and Applications (Hardcover, 2009 ed.)
Loot Price: R1,826
Discovery Miles 18 260
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Microinjection - Methods and Applications (Hardcover, 2009 ed.)
Series: Methods in Molecular Biology, 518
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As the number of sequenced genomes continues to increase,
understanding the functions of newly discovered molecules will
require greater efficiency and further study within the context of
live cells. In Microinjection: Methods and Applications, expert
researchers contribute methods utilizing microinjection techniques
ranging from expression of RNA to the integration of DNA into the
genome with the ultimate goal of learning about gene expression,
signal transduction, and protein function within these living
cells. This versatile volume updates established techniques such as
cRNA expression in Xenopus oocytes, and examines new, cutting-edge
technologies, including antisense morpholino oligonucleotides, RNAi
for knockdown experiments, and the use of integrase to produce
transgenic animals, all through microinjection techniques that can
be easily adopted by any lab. Written in the highly successful
Methods in Molecular Biology (TM) series format, chapters include
brief introductions to the topics, lists of the necessary materials
and reagents, step-by-step, readily reproducible laboratory
protocols, and Notes sections, highlighting tips on troubleshooting
and avoiding known pitfalls. Comprehensive and easily accessible,
Microinjection: Methods and Applications is an ideal source for new
ideas and techniques certain to aid in the advance of biological
research.
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