Modern Applications of DNA Amplification Techniques - Problems and New Tools (Paperback, 1997)

In the ten years since the first publication on PCR (Saiki et al. , 1985), this in vitro method of nucleic acid replication and modification has grown to rival in popularity traditional microbiological, genetical und technical procedures for cloning, sequencing, gene detecting and related procedures. To date the PCR literature has emphasized six main areas of application: genetic mapping, detection of mutations, genetic polymorphism, transcriptional splicing and regulation, molecular virology and quantitative procedures. The overwhelming focus of quantification of DNA or RNA by PCR has been on human microbiology and oncological problems. The exquisite sensitivity of PCR gives this method the ability to detect extremely rare DNAs, mRNAs, mRNAs in small numbers of cells or in small amounts of tissue, and mRNAs expressed in mixed-cell populations. However, the exact and accurate quantification of specific nucleic acids in biological samples is in spite of numerous publications in that field still a general problem: during the peR process, an unknown initial number of target sequences are used as a template from which a large quantity of specific product can be obtained. Although the amount of product formed is easy to determine, it is difficult to deduce the initial copy number of the target molecule because the efficiency of the peR is largely unknown.

General

Imprint:	Springer-Verlag New York
Country of origin:	United States
Release date:	April 2014
First published:	1997
Editors:	Dirk Lassner • Barbara Pustowoit • Arndt Rolfs
Dimensions:	254 x 178 x 8mm (L x W x T)
Format:	Paperback
Pages:	143
Edition:	1997
ISBN-13:	978-1-4613-7455-8
Categories:	Books > Science & Mathematics > Biology, life sciences > Zoology & animal sciences > General Books > Professional & Technical > Biochemical engineering > Biotechnology > General
LSN:	1-4613-7455-3
Barcode:	9781461374558

Is the information for this product incomplete, wrong or inappropriate? Let us know about it.

Does this product have an incorrect or missing image? Send us a new image.

Is this product missing categories? Add more categories.

Review This Product

No reviews yet - be the first to create one!