The exceptional organization of polytene chromosomes has made fruit
fly Drosophila melanogaster a premier model organism for
visualization of genes. A major barrier to full exploitation of
polytene chromosome cytology has been the difficulty in producing
good chromosome preparations. A new, economical procedure using
high pressure and other modifications to overcome this barrier is
presented. Light microscopy images of such slides compared to
electron microscopy images for the first time show similar or
superior detail. Results from typical staining applications,
including immunofluorescence and fluorescence in situ hybridization
are illustrated, and optimized protocols for light and electron
microscopy presented. Also included are notes on the general
architecture of polytene chromosomes. Improved chromosome images
prompted collaborative development of computer vision applications
for automated recognition and analysis of structural patterns.
These methods should be helpful for researchers and students using
the classical polytene chromosome model as well as to all
scientists exploring wonderful possibilities that computer vision
opens to modern microscopy.
General
| Imprint: |
VDM Verlag
|
| Country of origin: |
Germany |
| Release date: |
February 2009 |
| First published: |
February 2009 |
| Authors: |
Dmitri Novikov
|
| Dimensions: |
229 x 152 x 6mm (L x W x T) |
| Format: |
Paperback - Trade
|
| Pages: |
96 |
| ISBN-13: |
978-3-639-12924-3 |
| Categories: |
Books >
Science & Mathematics >
Biology, life sciences >
General
|
| LSN: |
3-639-12924-5 |
| Barcode: |
9783639129243 |
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