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Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice (Hardcover, 1st ed. 2018)
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Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice (Hardcover, 1st ed. 2018)
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This open access book presents simple, robust pre-field screening
protocols that allow plant breeders to screen for enhanced
tolerance to heat stress in rice. Two critical heat-sensitive
stages in the lifecycle of the rice crop are targeted - the
seedling and flowering stages - with screening based on simple
phenotypic responses. The protocols are based on the use of a
hydroponics system and/or pot experiments in a glasshouse in
combination with a controlled growth chamber where the heat stress
treatment is applied. The protocols are designed to be effective,
simple, reproducible and user-friendly. The protocols will enable
plant breeders to effectively reduce the number of plants from a
few thousands to less than 100 candidate individual mutants or
lines in a greenhouse/growth chamber, which can then be used for
further testing and validation in the field conditions. The methods
can also be used to classify rice genotypes according to their heat
tolerance characteristics. Thus, different types of heat stress
tolerance mechanisms can be identified, presenting opportunities
for pyramiding different (mutant) sources of heat stress tolerance.
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