Cardiovascular Proteomics: Methods and Protocols presents
cutting-edge protocols and strategies for proteomic evaluation of
cardiovascular disease written by pioneering researchers in the
field. Topics explored in this comprehensive volume include
obtaining specific heart proteins, cutting-edge techniques for
identifying risk biomarkers of atherome plaque rupture, analyzing
the secretome of explanted endarterectomies cultured in vitro, and
phage display techniques for deciphering the molecular diversity of
blood vessels. Detailed protocols for the isolation of short- and
long-term culture of the adult mouse cardiac myocytes are also
included.
Numerous advanced proteomic techniques are addressed, including
protein separation by two-dimensional electrophoresis and
two-dimensional difference gel electrophoresis, liquid
chromatography coupled to mass spectrometry, and the SELDI-TOF
approach to searching for biomarkers of stroke in human serum or of
hypertension in the serum of animal models. Proteomic strategies
and protocols for studying the proteome of endothelial, arterial
smooth muscle cells, foam cells, and circulating blood monocytes
constitute a major element of this text. Cutting-edge techniques
for the analysis of subproteomes from isolation to final
characterization and the characterization of posttranslational
modifications are also addressed. This useful snapshot of current
proteomic techniques is a single source for protocols for the
identification and validation of novel biomarkers and targets in
cardiovascular diseases.
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