This volume details technologies on recombinant DNA and nucleic
acid manipulation that underpin much of the biological sciences and
instructions on how to conduct them successfully. Chapters guide
the reader through the basics of oligonucleotide synthesis and DNA
sequencing; recombinant DNA plasmid work; cell-free experiments and
the latest developments in CRISPR approaches to genome
modification. Written in the format of the highly successful
Methods in Molecular Biology series, each chapter includes an
introduction to the topic, lists necessary materials and methods,
includes tips on troubleshooting and known pitfalls, and
step-by-step, readily reproducible protocols. Â Authoritative
and cutting-edge, Gene Modification and Nucleic Acid Technologies
aims to be the comprehensive guide for life scientists moving into
the field of recombinant DNA and nucleic acid manipulation.
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