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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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Epitope Mapping Protocols (Paperback, 1996 ed.)
Loot Price: R2,975
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Epitope Mapping Protocols (Paperback, 1996 ed.)
Series: Methods in Molecular Biology, 66
Expected to ship within 10 - 15 working days
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Interest in epitope mapping, or finding out where antibodies bind
to their antigens, is by no means restricted to immunologists, but
is shared by biolo gists from a wide range of disciplines in which
antibodies are used as molecu lar reagents. The epitope mapper may
be interested in studying protein-protein interactions, in
developing an immunoassay, in producing protective peptide
vaccines, in investigating the pathogenesis of autoimmune diseases,
or in defining protein topology in intact cells or organelles, to
mention only a few of the possibilities. The aim of Epitope Mapping
Protocols is to provide both a useful range of alternative
practical methods for the experienced mapper and a fairly com
prehensive introduction for someone embarking on antibody
production and mapping for the first time. Contributors were
encouraged to illustrate their protocols with results from their
own research and most of them elected to do so. After an
introductory chapter, the protocols are arranged in three groups:
The first group of twelve methods uses (or can use) whole, native
antigens and may therefore be suitable for conformational epitopes;
the second group of five uses peptides or peptide libraries; and
the third group of eleven requires antigen expressed from
recombinant DNA. Within this last group, methods more likely
applicable to conformational epitopes are placed first."
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