The last fifteen years have witnessed the birth and maturation
of many original methods and the development of protocols specific
to single molecule measurements and their analysis, including
techniques involving optical imaging, electron microscopy, optical
and magnetic trapping, and developments in atomic force microscopy.
In "Single Molecule Enzymology: Methods and Protocols," experts in
the field provide procedures which enable the extraction of
detailed information about enzyme work cycles, their static and
kinetic properties, and information about their location and
activity within cells. The detailed volume offers practical advice
on many aspects of single molecule enzymology and includes
strategic overviews of interconnected methods involved in sample
preparation, single molecule measurements, and data analysis.
Written in the highly successful "Methods in Molecular Biology "
series format, chapters contain introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, "Single Molecule Enzymology:
Methods and Protocols" is intended for use within the diverse
community of molecular biologists, biochemists, and biophysicists
studying enzymes in detail and can be used by researchers planning
their first single molecule study or to aid more experienced
researchers in further developing their existing studies."
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