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Programmed Cell Death - Methods and Protocols (Hardcover, 1st ed. 2016)
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Programmed Cell Death - Methods and Protocols (Hardcover, 1st ed. 2016)
Series: Methods in Molecular Biology, 1419
Expected to ship within 12 - 17 working days
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This volume contains protocols specifically designed for studying
programmed cell death, and also discusses recent advances in
techniques that span broader areas of biology that have been
recently used or that have potential to be incorporated into cell
death research. The protocols are mostly described in the context
of mammalian systems, but also cover other systems such as plants,
Drosophila, and yeast. Programmed Cell Death: Methods and Protocols
is comprised of 20 chapters: Chapters 1-5 describe apoptosis
detection techniques; Chapter 6-9 describe methods for studying
apoptosis associated with various pathologies in different organs
including the lymphoid compartment, intestinal epithelium,
granulocytes, and cardiomyocytes; Chapter 11-13 cover protocols and
techniques for studying apoptosis in non-mammalian systems;
Chapters 14-16 cover biochemical and biophysical methods for
studying Bcl-2 family protein dynamics and protein-protein
interactions during apoptosis; and the last four chapters explore
protocols that are useful not only in apoptosis research but in
wider areas of biological research, such as genome editing,
inducible transgenes, and proteomics. Written in the highly
successful Methods in Molecular Biology aeries format, chapters
include introductions to their respective topics, lists of the
necessary material and reagents, step-by-step, readily reproducible
laboratory protocol, and tips on troubleshooting and avoiding known
pitfalls. Thorough and cutting-edge, Programmed Cell Death: Methods
and Protocols is a comprehensive and valuable resource for
researchers, ranging from beginner to expert, in their studies on
programmed cell death.
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