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Mammalian Oocyte Regulation - Methods and Protocols (Hardcover, 2013 ed.)
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Mammalian Oocyte Regulation - Methods and Protocols (Hardcover, 2013 ed.)
Series: Methods in Molecular Biology, 957
Expected to ship within 12 - 17 working days
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Mammalian oocytes occupy a critical nexus in reproduction as they
not only contribute half the genetic makeup of the embryo but also
provide virtually all of the cytoplasmic building blocks required
for sustaining embryogenesis. The journey that transforms a
primordial germ cell into a mature oocyte (or egg) capable of
fertilisation and embryonic development is of unrivalled
complexity. The end-product of this remarkable developmental
process is the "mature" egg, now replete with macromolecules and
organelles, and poised to support embryonic development if
fertilisation should occur. Given the importance of the oocyte for
embryonic development, it is of no surprise that the oocyte holds
centre stage in fertility clinics and greatly influences the
outcome of assisted reproductive treatments (ARTs). Mammalian
Oocyte Regulation: Methods and Protocols provides a highly diverse
compendium of detailed methodologies - primarily focusing on the
murine model but also including chapters on human oocytes - for
enabling researchers to interrogate every aspect of mammalian
oocyte development including recombination, meiotic maturation,
oocyte substrate uptake, chromosomal segregation and fertilisation.
Written in the highly successful Methods in Molecular Biology (TM)
series format, chapters contain introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Authoritative and
easily accessible, Mammalian Oocyte Regulation: Methods and
Protocols serves as an ideal guide to novice oocyte researchers,
experts who wish to add another dimension to their established
portfolio, as well as the wider research community.
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