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Cytochrome P450 Protocols (Hardcover, 2nd ed. 2006)
Loot Price: R4,276
Discovery Miles 42 760
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Cytochrome P450 Protocols (Hardcover, 2nd ed. 2006)
Series: Methods in Molecular Biology, 320
Expected to ship within 12 - 17 working days
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For this second edition of their much praised Cytochrome P450, the
editors have collected accounts of the essential core techniques
that use the latest methodologies for the investigation of P450s.
Highlights include protocols for spectral analysis and purification
of P450s, enzymatic assays of P450s and flavin-containing
monooxygenases (FMOs), expression of P450s and FMOs in heterologous
systems, and the production and use of antipeptide antibodies.
Additional chapters contain readily reproducible techniques for the
transfection of hepatocytes for gene regulation studies, P450
reporter gene assays, in situ hybridization, and analysis of
genetic polymorphisms. Although the emphasis is on P450s of
mammalian origin, many of the readily reproducible methods
described are suitable for P450s from any source.
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