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Transgenic Crops of the World - Essential Protocols (Paperback, Softcover reprint of the original 1st ed. 2004)
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Transgenic Crops of the World - Essential Protocols (Paperback, Softcover reprint of the original 1st ed. 2004)
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Since the first transgenic plants were produced back in the early
1980s, there have been substantial developments towards the genetic
engineering of most crops of our world. Initial studies using
isolated plant cells and removing their cell walls to form
protoplasts, offered the possibility of transferring genetic
material by Agrobacterium-mediated gene transfer, chemical agents
or electrical charges. However, in those cases were isolated
protoplasts could be transformed, often, a shoot regeneration
system was not available to induce the production of transgenic
plants and any such regenerated plants were subject to mutation or
chromosomal of cultured plant organs, such as leaf abnormalities.
By the mid-1980s, the use disks, offered the convenience of
combining gene transfer, plant regeneration and selection of
transformants in a single system. This approach, enabled the
production of stable, phenotypically-normal, transgenic potato and
tomato plants in culture. By the late 1980s, the use of biolistics
offered a means of inserting foreign genes into plant cells which
where inaccessible to Agrobacterium infection. Even today, this
technology is now standard practice for the production of some
transgenic plants.
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