Membrane proteins play a key role in numerous pathologies such
as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and
Alzheimer s disease, yet studies on these and other disorders are
hampered by a lack of information about the proteins involved. In
Heterologous Expression of Membrane Proteins: Methods and
Protocols, expert researchers provide an overview of the different
heterologous expression systems available to produce these proteins
for structural analysis, helping us to understand how these
proteins function, and additionally, how their functions can be
modified through drug treatment. Chapters examine membrane protein
solubilization, purification, and instability in solution, and
investigate the strategies that helped to determine the structure
of the first heterologously expressed mammalian membrane proteins.
Composed in the highly successful Methods in Molecular Biology
series format, each chapter contains a brief introduction,
step-by-step methods, a list of necessary materials, and a Notes
section which shares tips on troubleshooting and avoiding known
pitfalls.
Far-reaching and innovative, Heterologous Expression of Membrane
Proteins: Methods and Protocols, is an essential guide for novice
and experienced researchers alike, providing detailed methods for
existing expression systems alongside cutting-edge strategies for
future use in the field."
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