This book focuses on recent developments of Pichia pastoris as a
recombinant protein production system. Highlighted topics include a
discussion on the use of fermentors to grow Pichia pastoris,
information on the O- and N-linked glycosylation, methods for
labeling Pichia pastoris expressed proteins for structural studies,
and the introduction of mutations in Pichia pastoris genes by the
methods of restriction enzyme-mediated integration (REMI). Each
chapter presents cutting-edge and cornerstone protocols for
utilizing P. pastoris as a model recomibinant protein production
system. This volume fully updates and expands upon the first
edition.
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