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DNA Arrays - Methods and Protocols (Hardcover, 2001 ed.)
Loot Price: R3,094
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DNA Arrays - Methods and Protocols (Hardcover, 2001 ed.)
Series: Methods in Molecular Biology, 170
Expected to ship within 10 - 15 working days
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Microarray technology provides a highly sensitive and precise te-
nique for obtaining information from biological samples, with the
added advantage that it can handle a large number of samples
simultaneously that may be analyzed rapidly. Researchers are
applying microarray technology to understand gene expression,
mutation analysis, and the sequencing of genes. Although this
technology has been experimental, and thus has been through
feasibility studies, it has just recently entered into widespread
use for advanced research. The purpose of DNA Arrays: Methods and
Protocols is to provide instruction in designing and constructing
DNA arrays, as well as hybridizing them with biological samples for
analysis. An additional purpose is to p- vide the reader with a
broad description of DNA-based array technology and its potential
applications. This volume also covers the history of DNA
arrays-from their conception to their ready off-the-shelf
availability-for readers who are new to array technology as well as
those who are well versed in this field. Stepwise, detailed
experimental procedures are described for constructing DNA arrays,
including the choice of solid support, attachment methods, and the
general conditions for hybridization. With microarray technology,
ordered arrays of oligonucleotides or other DNA sequences are
attached or printed to the solid support using au- mated methods
for array synthesis. Probe sequences are selected in such a way
that they have the appropriate sequence length, site of mutation,
and T .
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