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Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana - Protocols (Paperback, 1st ed. 2022)
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Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana - Protocols (Paperback, 1st ed. 2022)
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Bananas are a staple food for over 500 million people and are also
an important cash crop. Fusarium wilt, caused by the fungus
Fusarium oxysporum f.sp. cubense, is one of the most destructive
diseases of banana globally. Since the 1990s, an aggressive variant
of this fungus, called Tropical Race 4 (TR4), severely affected
banana plantations in Southeast Asia from where it spread to other
continents, including Latin America, where the global banana export
market is primarily centred. TR4 is a soil borne pathogen making
the disease difficult to contain. The Joint FAO/IAEA Centre of
Nuclear Techniques in Food and Agriculture implemented a
Coordinated Research Project (CRP) 'Efficient Screening Techniques
to Identify Mutants with Disease Resistance for Coffee and Banana"
(2015-2020). This CRP brought together experts from Asia, Europe
and Africa in addition to experts of the Joint FAO/IAEA Centre to
develop resistance against TR4 through mutation-assisted breeding.
Induced mutagenesis is particularly attractive in case of banana
since most cultivated bananas are seedless, thus hampering
conventional cross breeding. This Open Access book is a compilation
of the protocols developed under the CRP specifically for TR4. The
first part covers methods for mutation induction, including the
integrated use of innovative single-cell culture with mutagenesis
techniques. The book also describes up-to-date phenotypic screening
methods for TR4 resistance in banana under field-, greenhouse- and
laboratory conditions. Finally, molecular and bioinformatics tools
for genome-wide mutation discovery following Next Generation
Sequencing are also described. Given the imminent threat of
Fusarium Wilt TR4 on banana production globally, it is our hope and
intention that the book will serve as a timely reference and guide
for banana breeders and pathologists worldwide who are committed to
the genetic improvement of banana for Fusarium wilt resistance.
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