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Applications of Continuous and Steady-State Methods to Root Biology (Paperback, Softcover reprint of the original 1st ed. 1989)
Loot Price: R4,433
Discovery Miles 44 330
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Applications of Continuous and Steady-State Methods to Root Biology (Paperback, Softcover reprint of the original 1st ed. 1989)
Series: Developments in Plant and Soil Sciences, 34
Expected to ship within 10 - 15 working days
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This book provides an excellent illustration of the
interrelationship between progress in scientific methodology and
conceptual advances, and its publica tion should contribute to
further advances. It is well known that major advances in
understanding often follow the development of new methods. The
development of the acetylene reduction assay for nitrogenase
activity provides a good example of this interrelationship between
theory and methods. Theoretical knowledge led to a search for
substrates for nitro genase that could be assayed for more easily
than ammonium, the normal product of the enzyme. The discovery of
the reduction of acetylene to ethylene by nitrogenase provided the
ideal answer to the problem by provid ing a rapid, specific,
nondestructive, and inexpensive assay for nitrogenase activity.
This assay is now used by almost every laboratory doing research on
nitrogen fixation. However, further use and development of the
acetylene reduction assay has shown that it can underestimate
nitrogenase activity and can even give incorrect relative values
under some circumstances. The major problem is that exposure of
legume nodules to acetylene can cause a large increase in the
resistance to oxygen diffusion into the nodule. This reduced supply
of oxygen decreases the rate of nitrogenase activity within a few
minutes."
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