In "Functional Analysis of DNA and Chromatin," expert
researchers in the field provide an overview of standard and more
recent methods for the functional analysis of the genetic material.
These include methods on DNA-dye binding modes, chromatin staining,
nuclear dispersion assays, DNA labeling" in vivo," sister chromatid
exchanges, FISH, DNA and chromatin imaging by fluorescence,
electron and atomic force microscopy, detection of apoptotic DNA,
cytosine methylation and hydroxy-methylation, DNA thermophoresis,
improved methods for histone analysis, chromatin
immunoprecipitation, and analysis of rDNA genes and
chromatin-associated RNA. Written in the highly successful "Methods
in Molecular Biology "series format, chapters include introductions
to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and key tips on troubleshooting and avoiding known pitfalls.
Authoritativeand practical, "Functional Analysis of DNA and
Chromatin "seeks to aid scientists in the further study of cellular
and molecular biology of the nucleus.
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