Plant virus and sub-viral pathogens pose severe constraints to
the production of wide range of economically important crops
worldwide. The crops raised both through true seed and vegetative
propagated materials are affected with number of virus and
virus-like diseases. The virus may enter into plants through seed
planting materials or by vectors. Once the virus is in the field,
it multiplies and spreads following definite patterns depending
upon the nature of the vector and agro-meteorological conditions.
Disease free crops and plants are great economic and social
importance in feeding the world's population. Detection of virus
and sub-viral agents at initial stages of infection is critical to
reduce economic losses. For nearly two decades, ELISA and its
variants played a major role in large scale virus testing and also
in the production of virus-free planting materials.
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