Gene Essentiality - Methods and Protocols (Hardcover, 2015 ed.)

This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 1279
Release date:	2015
First published:	2015
Editors:	Long Jason Lu
Dimensions:	254 x 178 x 16mm (L x W x T)
Format:	Hardcover - Cloth over boards
Pages:	248
Edition:	2015 ed.
ISBN-13:	978-1-4939-2397-7
Categories:	Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > General Promotions
LSN:	1-4939-2397-8
Barcode:	9781493923977

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