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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Lipids
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Lipid Biochemistry - An introduction (Paperback, Softcover reprint of the original 1st ed. 1991)
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Lipid Biochemistry - An introduction (Paperback, Softcover reprint of the original 1st ed. 1991)
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Lipids can usually be extracted easily from tissues by making use
of their hydrophobic characteristics. However, such extractions
yield a complex mixture of different lipid classes which have to be
purified further for quantitative analysis. Moreover, the crude
lipid extract will be contami nated by other hydrophobic molecules,
e.g. by intrinsic membrane proteins. Of the various types of
separation processes, thin layer and column chromatography are most
useful for intact lipids. High performance liquid chromatography
(HPLC) is also rapidly becoming more popular, especially for the
fractionation of molecular species of a given lipid class. The most
powerful tool for quantitation of the majority of lipids is gas
liquid chromatography (GLC). The method is very sensitive and, if
adapted with capillary columns, can provide information with regard
to such subtle features as the position or configuration of
substitutions along acyl chains. By coupling GLC or HPLC to a
radioactivity detector, then the techniques are also very useful
for metabolic measurements. Although research laboratories use
generally sophisticated analytical methods such as GLC to analyse
and quantify lipid samples, chemical derivatii: ations are often
used in hospitals. For these methods, the lipid samples are
derivatized to yield a product which can be measured simply and
accurately"- usually by colour. Thus, total triacylglycerol,
cholesterol or phospholipid-phosphorus can be quantitated
conveniently without bothering with the extra information of
molecular species, etc. which might be determined by more thorough
analyses. REFERENCES Christie, W.W. (1982) Lipid Analysis, 2nd edn,
Pergamon Press, Oxford."
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