The work presented in this book consists of three disparate
elements which have the unifying feature of being concerned with
alphasatellite. The satellite-like alphasatellites (previously
known as DNA 1) were first identified in 1999 and were shown to be
associated with the majority of begomoviruses (family
Geminiviridae) that require the satellite molecule now collectively
known as betasatellites. Virus-induced gene silencing (VIGS) is a
powerful reverse genetics tool for application in functional
genomics. The alphasatellite-based VIGS vector was shown to be
functional by silencing expression of GFP in 16c plants and
magnesium chelatase. In addition, the alphasatellite vector was
shown to be useful as a gene expression vector. An analysis of
tomato plants from Oman exhibiting severe leaf curl symptoms showed
the presence of Tomato yellow leaf curl-Oman (TYLCV-Om), a virus
previously shown to be present in tomato in this country, a new
recombinant begomovirus species, for which the name Tomato leaf
curl Oman virus (ToLCOMV) is proposed, a betasatellite (tobacco
leaf curl betasatellite (TbLCB) and an unusual alphasatellite. The
significance of these findings is discussed.
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